Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/ijms24087336
Keywords
isatin-derived spirooxindoles (SOXs); cancer cells; drug-likeness; ADMET studies; ROS generation; and anticancer effects
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A highly stereo-selective, one-pot, multicomponent method was used to synthesize novel functionalized 1,3-cycloaddition spirooxindoles (SOXs) (4a-4h). The synthesized SOXs were analyzed for drug-likeness, ADME parameters, and anticancer activity. Molecular docking analysis revealed that SOX 4a had substantial binding affinity with CD-44, EGFR, AKR1D1, and HER-2, and functional studies showed that SOX 4a induced cell death in cancer cells by affecting cytoplasmic and nuclear architecture and inducing ROS generation.
A highly stereo-selective, one-pot, multicomponent method was chosen to synthesize the novel functionalized 1, 3-cycloaddition spirooxindoles (SOXs) (4a-4h). Synthesized SOXs were analyzed for their drug-likeness and ADME parameters and screened for their anticancer activity. Our molecular docking analysis revealed that among all derivatives of SOXs (4a-4h), 4a has a substantial binding affinity ( increment G) -6.65, -6.55, -8.73, and -7.27 Kcal/mol with CD-44, EGFR, AKR1D1, and HER-2, respectively. A functional study demonstrated that SOX 4a has a substantial impact on human cancer cell phenotypes exhibiting abnormality in cytoplasmic and nuclear architecture as well as granule formation leading to cell death. SOX 4a treatment robustly induced reactive oxygen species (ROS) generation in cancer cells as observed by enhanced DCFH-DA signals. Overall, our results suggest that SOX (4a) targets CD-44, EGFR, AKR1D1, and HER-2 and induces ROS generation in cancer cells. We conclude that SOX (4a) could be explored as a potential chemotherapeutic molecule against various cancers in appropriate pre-clinical in vitro and in vivo model systems.
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