Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/ijms24119550
Keywords
ATR-IR spectroscopy; spike glycoproteins; secondary structure; conformation; SARS-CoV; MERS-CoV; SARS-CoV-2
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In this study, the conformation of the S1 subunits of MERS-CoV, SARS-CoV, and SARS-CoV-2 were investigated by measuring their Amide I infrared absorption bands. The results showed significant differences in the S1 secondary structure of SARS-CoV-2 compared to MERS-CoV and SARS-CoV, including a significant presence of extended beta-sheets. Additionally, the SARS-CoV-2 S1 conformation exhibited significant changes in mild acidic and alkaline pH conditions compared to serological pH. These findings suggest the potential of infrared spectroscopy in studying the secondary structure adaptation of SARS-CoV-2 S1 in different environments.
All coronaviruses are characterized by spike glycoproteins whose S1 subunits contain the receptor binding domain (RBD). The RBD anchors the virus to the host cellular membrane to regulate the virus transmissibility and infectious process. Although the protein/receptor interaction mainly depends on the spike's conformation, particularly on its S1 unit, their secondary structures are poorly known. In this paper, the S1 conformation was investigated for MERS-CoV, SARS-CoV, and SARS-CoV-2 at serological pH by measuring their Amide I infrared absorption bands. The SARS-CoV-2 S1 secondary structure revealed a strong difference compared to those of MERS-CoV and SARS-CoV, with a significant presence of extended beta-sheets. Furthermore, the conformation of the SARS-CoV-2 S1 showed a significant change by moving from serological pH to mild acidic and alkaline pH conditions. Both results suggest the capability of infrared spectroscopy to follow the secondary structure adaptation of the SARS-CoV-2 S1 to different environments.
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