Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms24076726
Keywords
long glume; Psathyrostachys huashanica Keng; molecular cytogenetics; RNA-seq; marker development
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In this study, the wheat-P. huashanica derived line 20JH1155 was found to contain 21 pairs of wheat chromosomes and a pair of P. huashanica chromosomes through cytological observation, GISH, and sequential FISH analysis. Transcriptome sequencing revealed differentially expressed genes during immature spikelet development of 20JH1155 and 7182, as well as predicted transcription factors. Additionally, molecular markers derived from Ph#3Ns were developed from transcriptome data. Overall, the wheat-P. huashanica derived line 20JH1155 provides new opportunities for distant hybridization of wheat and accelerates the utilization of genes from P. huashanica.
Psathyrostachys huashanica Keng (2n = 2x = 14, NsNs) is an excellent gene resource for wheat breeding, which is characterized by early maturity, low plant height, and disease resistance. The wheat-P. huashanica derivatives were created by the elite genes of P. huashanica and permeate into common wheat through hybridization. Among them, a long-glume material 20JH1155 was identified, with larger grains and longer spike than its parents. In the present study, the methods of cytological observation, GISH, and sequential FISH analysis showed that 20JH1155 contained 21 pairs of wheat chromosomes and a pair of P. huashanica. There were some differences in 5A and 7B chromosomes between 20JH1155 and parental wheat 7182. Molecular marker, FISH, and sequence cloning indicated 20JH1155 alien chromosomes were 3Ns of P. huashanica. In addition, differentially expressed genes during immature spikelet development of 20JH1155 and 7182 and predicted transcription factors were obtained by transcriptome sequencing. Moreover, a total of 7 makers derived from Ph#3Ns were developed from transcriptome data. Taken together, the wheat-P. huashanica derived line 20JH1155 provides a new horizon on distant hybridization of wheat and accelerates the utilization of genes of P. huashanica.
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