Development of a Novel, Automated High-Throughput Device for Performing the Comet Assay

A comet assay is a trusted and widely used method for assessing DNA damage in individual eukaryotic cells. However, it is time-consuming and requires extensive monitoring and sample manipulation by the user. This limits the throughput of the assay, increases the risk of errors, and contributes to intra- and inter-laboratory variability. Here, we describe the development of a device which automates high throughput sample processing for a comet assay. This device is based upon our patented, high throughput, vertical comet assay electrophoresis tank, and incorporates our novel, patented combination of assay fluidics, temperature control, and a sliding electrophoresis tank to facilitate sample loading and removal. Additionally, we demonstrated that the automated device performs at least as well as our manual high throughput system, but with all the advantages of a fully walkaway device, such as a decreased need for human involvement and a decreased assay run time. Our automated device represents a valuable, high throughput approach for reliably assessing DNA damage with the minimal operator involvement, particularly if combined with the automated analysis of comets.

Automated summary

This article describes the development of an automated device for high throughput sample processing in a comet assay, which reliably assesses DNA damage in individual eukaryotic cells. The device performs as well as manual methods but with the advantages of decreased human involvement and assay run time, making it a valuable approach for high throughput DNA damage assessment.