Electrochemical Immunosensor for the Determination of Antibodies against Prostate-Specific Antigen Based on ZnO Nanostructures

In this study, ZnO nanostructures with different types of morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The first material was composed of spherical, polydisperse nanostructures with a particle size in the range of 10-160 nm. The second was made up of more compact rod-like spherical nanostructures with the diameter of these rods in the range of 50-400 nm, and approximately 98% of the particles were in the range of 20-70 nm. The last sample of ZnO was made up of rod-shaped particles with a diameter of 10-80 nm. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes (SPCE), followed by a further immobilization of the prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA (anti-PSA) was evaluated using the differential pulse voltammetry technique. The limit of detection and limit of quantification of anti-PSA were determined as 1.35 nM and 4.08 nM for compact rod-shaped spherical ZnO nanostructures, and 2.36 nM and 7.15 nM for rod-shaped ZnO nanostructures, respectively.

Automated summary

ZnO nanostructures with different morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The nanostructures included spherical polydisperse structures, compact rod-like spherical structures, and rod-shaped particles. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes, followed by immobilization of prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA was evaluated using the differential pulse voltammetry technique. The detection and quantification limits for anti-PSA were determined for different ZnO nanostructures.