Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ijms24065803
Keywords
zinc oxide nanostructures; one-dimensional nanostructures; electrochemical immunosensor; cyclic voltammetry; differential pulse voltammetry; antigen-antibody complex
Ask authors/readers for more resources
ZnO nanostructures with different morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The nanostructures included spherical polydisperse structures, compact rod-like spherical structures, and rod-shaped particles. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes, followed by immobilization of prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA was evaluated using the differential pulse voltammetry technique. The detection and quantification limits for anti-PSA were determined for different ZnO nanostructures.
In this study, ZnO nanostructures with different types of morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The first material was composed of spherical, polydisperse nanostructures with a particle size in the range of 10-160 nm. The second was made up of more compact rod-like spherical nanostructures with the diameter of these rods in the range of 50-400 nm, and approximately 98% of the particles were in the range of 20-70 nm. The last sample of ZnO was made up of rod-shaped particles with a diameter of 10-80 nm. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes (SPCE), followed by a further immobilization of the prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA (anti-PSA) was evaluated using the differential pulse voltammetry technique. The limit of detection and limit of quantification of anti-PSA were determined as 1.35 nM and 4.08 nM for compact rod-shaped spherical ZnO nanostructures, and 2.36 nM and 7.15 nM for rod-shaped ZnO nanostructures, respectively.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available