Chitosan as a Promising Support of a CDH Activity Preservation System for Biomedical and Industrial Applications

Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoprotein catalyzing the oxidation reaction of beta-1,4-glycosidic-bonded sugars (lactose or cellobiose), which results in the formation of aldobionic acids and hydrogen peroxide as a byproduct. The biotechnological application of CDH requires the immobilization of the enzyme on a suitable support. As a carrier of natural origin used for CDH immobilization, chitosan seems to increase the catalytic potential of the enzyme, especially for applications as packaging in the food industry and as a dressing material in medical applications. The present study aimed to immobilize the enzyme on chitosan beads and determine the physicochemical and biological properties of immobilized CDHs obtained from different fungal sources. The chitosan beads with immobilized CDHs were characterized in terms of their FTIR spectra or SEM microstructure. The most effective method of immobilization in the proposed modification was the covalent bonding of enzyme molecules using glutaraldehyde, resulting in efficiencies ranging from 28 to 99%. Very promising results, compared to free CDH, were obtained in the case of antioxidant, antimicrobial, and cytotoxic properties. Summarizing the obtained data, chitosan seems to be a valuable material for the development of innovative and effective immobilization systems for biomedical applications or food packaging, preserving the unique properties of CDH.

Automated summary

The study aimed to immobilize cellobiose dehydrogenase (CDH) on chitosan beads and characterize the properties of immobilized CDHs from different fungal sources. The immobilized CDHs were characterized using FTIR spectra and SEM microstructure. The most effective method of immobilization was the covalent bonding of enzyme molecules using glutaraldehyde, showing efficiencies ranging from 28% to 99%. Promising results were obtained in terms of antioxidant, antimicrobial, and cytotoxic properties compared to free CDH. Chitosan appears to be a valuable material for the development of immobilization systems for biomedical applications or food packaging, while preserving the unique properties of CDH.