Journal
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Volume 128, Issue -, Pages 128-131Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2022.12.038
Keywords
Metagenomic next-generation sequencing; Spirometra mansoni; Infection
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A 30-year-old male patient presented with a cyst on the left hip that had been progressively enlarging for over 2 months. Initial investigations pointed towards a cysticercosis infection, but treatment was ineffective. Subsequent metagenomic next-generation sequencing (mNGS) analysis of surgically excised tissue revealed a Spirometra mansoni infection, which was further confirmed by polymerase chain reaction and phylogenetic analysis. This study highlights the superior diagnostic capability of mNGS in identifying parasitic infections.
A 30-year-old male patient had a cyst on the left hip and progressive enlargement for more than 2 months. Combined blood tests, magnetic resonance imaging, and pathology findings, cysticercosis infec-tion was suspected. However, the treatment for cysticercosis was ineffective. We conducted a metage-nomic next-generation sequencing (mNGS) analysis on the formalin-fixed, paraffin-embedded specimen of the patient's surgically excised tissue, and the results suggested Spirometra mansoni, mNGS was fur-ther confirmed by polymerase chain reaction and phylogenetic analysis of cytochrome c oxidase subunit 1 (cox1) gene. Based on these results, we found that mNGS provided a better method of diagnosing par-asitic infections.(c) 2022 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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