Journal
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
Volume -, Issue -, Pages -Publisher
WILEY
DOI: 10.1111/1471-0307.12978
Keywords
Ampicillin; Amoxicillin; Antibiotic resistance; Whole genome sequencing; Mutation
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This study evaluated the development of ampicillin and amoxicillin resistance in Lactobacillus plantarum ATCC14917 during laboratory evolution. Both evolved strains exhibited high levels of resistance in ampicillin and amoxicillin environments. While resistance to amoxicillin returned to a sensitive level after subsequent passage in antibiotic-free medium, resistance to ampicillin remained stable. Homology-based analysis indicated that the spread of antibiotic resistance genes in the gut would not occur with these mutant genes. This study provides evidence supporting the safe use of L. plantarum ATCC14917 in clinical practice when ampicillin and amoxicillin are used concurrently.
This study assessed the ampicillin and amoxicillin resistance in Lactobacillus plantarum ATCC14917 during the laboratory evolution in culture medium, including a gradually increasing concentration of antibiotic. In ampicillin and amoxicillin environment, both evolved strains obtained high levels of resistance. After the subsequent passage in antibiotic-free medium, amoxicillin-resistant strains returned to sensitive level, but resistance to ampicillin remained stable. The homology-based analysis showed that the spread of antibiotic resistance genes in the gut would not occur in these mutant genes. This study could serve as evidence supporting the safe use of L. plantarum ATCC14917 in clinical practice when ampicillin and amoxicillin were concurrently used.
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