C9 regulates the complement-mediated cell lysis in association with CD59 to resist bacterial infection in a primary animal

Complement component 9 (C9), as an essential component of terminal membrane attack complex of complement system, plays an important role in innate immune defense. However, the function and regulatory mechanism of C9 in the antimicrobial immune response of teleost fish remain unclear. In this study, the open reading frame of Nile tilapia (Oreochromis niloticus) C9 (OnC9) gene was amplified. The mRNA and protein expression of OnC9 were significantly changed upon infection with Streptococcus agalactiae and Aeromonas hydrophila in vivo and in vitro. Upon bacterial challenge, the OnC9 knockdown could lead to rapid proliferation of the pathogenic bacteria, ultimately resulting in tilapia death. However, the phenotype was rescued by re-injection of OnC9, which restored the healthy status of the knockdown tilapia. Further, the OnC9 was an essential component in complement-mediated cell lysis and associated with OnCD59 to regulate the efficiency of lysis. Overall, this study indicates that OnC9 is involved in host defense against bacterial infection, and provides a valuable reference for further exploration of the molecular regulatory mechanism of C9 in innate immune defense in a primary animal.

Automated summary

This study explores the role of complement component 9 (C9) in the immune response of tilapia fish to bacterial infection. The study found that the expression of OnC9 was significantly changed upon infection and knockdown of OnC9 led to rapid proliferation of pathogenic bacteria and fish death. However, the phenotype was rescued by re-injection of OnC9. The study also reveals that OnC9 is involved in complement-mediated cell lysis and is associated with OnCD59 to regulate the efficiency of lysis.