Study of anti-fatigue activity of polysaccharide from fruiting bodies of Armillaria gallica

Fatigue is a common physiological response that is closely related to energy metabolism. Polysaccharides, as excellent dietary supplements, have been proven to have a variety of pharmacological activities. In this study, A 23.007 kDa polysaccharide from Armillaria gallica (AGP) was purified and performed structural characterization, including analysis of homogeneity, molecular weight and monosaccharide composition. Methylation analysis is used to analyze the glycosidic bond composition of AGP. The mouse model of acute fatigue was used to evaluate the anti-fatigue effect of AGP. AGP-treatment improved exercise endurance in mice and reduced fatigue symp-toms caused by acute exercise. AGP regulated the levels of adenosine triphosphate, lactic acid, blood urea ni-trogen and lactate dehydrogenase, muscle glycogen and liver glycogen of acute fatigue mice. AGP affected the composition of intestinal microbiota, the changes of some intestinal microorganisms are correlated with fatigue and oxidative stress indicators. Meanwhile, AGP reduced oxidative stress levels, increased antioxidant enzyme activity and regulated the AMP-dependent protein kinase/nuclear factor erythroid 2-related factor 2 signaling pathway. AGP exerted an anti-fatigue effect through modulation of oxidative stress, which is related to intestinal microbiota.

Automated summary

In this study, a polysaccharide (AGP) was extracted and purified from Armillaria gallica. The structural characterization of AGP was performed, including analysis of homogeneity, molecular weight and monosaccharide composition. AGP exhibited anti-fatigue effects in a mouse model of acute fatigue, improving exercise endurance and reducing fatigue symptoms. AGP also regulated various biochemical parameters related to fatigue, affected the composition of intestinal microbiota, and modulated oxidative stress levels through the AMP-dependent protein kinase/nuclear factor erythroid 2-related factor 2 signaling pathway.