Silver sulfide coated alginate radioenhancer for enhanced X-ray radiation therapy of breast cancer

A wide range of high-Z nanomaterials are fabricated to decrease radiation dose by sensitizing cells to irradiation through various mechanisms such as ROS generation enhancement. Alginate-coated silver sulfide nanoparticles (Ag2S@Alg) were synthesized and characterized by SEM, TEM, DLS, XRD, EPS, FT-IR, and UV-vis analysis techniques. Cytotoxicity of nanoparticles was tested against HFF-2, MCF-7, and 4 T1 cell lines for biocompati-bility and radio enhancement ability evaluation, respectively. Moreover, the hemolysis assay demonstrated that the nanoparticles were biocompatible and nontoxic. In vitro intracellular ROS generation and calcein AM/PI co -staining unveiled cancerous cell death induction by nanoradiosensitizer, Ag2S@Alg. Further, histopathology results emphasized the tumor ablation capability of Ag2S@Alg. Silver anticancer properties were also recognized and combined with its radiosensitizing effect under X-ray irradiation.

Automated summary

A wide range of high-Z nanomaterials, including Alginate-coated silver sulfide nanoparticles (Ag2S@Alg), have been developed for their capability to sensitize cells to irradiation by enhancing ROS generation. Various analysis techniques were used to characterize the synthesized Ag2S@Alg nanoparticles. The cytotoxicity test and hemolysis assay confirmed the biocompatibility and non-toxicity of the nanoparticles. In vitro experiments demonstrated the induction of cancer cell death and tumor ablation capability of Ag2S@Alg, along with its radiosensitizing effect under X-ray irradiation.