Immediate-sustained lactate release using alginate hydrogel assembled to proteinase K/polymer electrospun fibers

This work proposes a microfibers-hydrogel assembled composite as delivery vehicle able to combine into a single system both burst and prolonged release of lactate. The prolonged release of lactate has been achieved by electrospinning a mixture of polylactic acid and proteinase K (26.0 mg of proteinase K and 0.99 g of PLA dis -solved in 6 mL of 2:1 chloroform:acetone in the optimal case), which is a protease that catalyzes the degradation of polylactic acid into lactate. The degradation of microfibers into lactate reflects that proteinase K preserves its enzymatic activity even after the electrospinning process because of the mild operational conditions used. Be -sides, burst release is obtained from the lactate-loaded alginate hydrogel. The successful assembly between the lactate-loaded hydrogel and the polylactic acid/proteinase K fibers has been favored by applying a low-pressure (0.3 mbar at 300 W) oxygen plasma treatment, which transforms hydrophobic fibers into hydrophilic while the enzymatic activity is still maintained. The composite displays both fast (< 24 h) and sustained (> 10 days) lactate release, and allows the modulation of the release by adjusting either the amount of loaded lactate or the amount of active enzyme.

Automated summary

This study presents the development of a microfibers-hydrogel assembled composite that can achieve both burst and prolonged release of lactate in a single system. The prolonged release is achieved by electrospinning a mixture of polylactic acid and proteinase K, which catalyzes the degradation of polylactic acid into lactate. Burst release is obtained from the lactate-loaded alginate hydrogel. The successful assembly between the hydrogel and the polylactic acid/proteinase K fibers is facilitated by low-pressure oxygen plasma treatment.