4.7 Article

Design of a High-Throughput Robotic Batch Microinjection System for Zebrafish Larvae-Based on Image Potential Energy

Related references

Note: Only part of the references are listed.
Article Automation & Control Systems

Design and Development of a Dexterous Bilateral Robotic Microinjection System Based on Haptic Feedback

Kai Feng et al.

Summary: This paper presents the design and development of a dexterous bilateral robotic system with haptic feedback for microinjection of zebrafish larvae and embryos. The system exhibits multi-DOF motion and enables remote control and intuitive haptics feedback, leading to a high success rate in microinjection operations.

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING (2023)

Article Instruments & Instrumentation

Posture adjustment and robust microinjection of zebrafish larval heart

Qigao Fan et al.

Summary: This paper presents an automated microinjection system for zebrafish hearts, addressing the challenges posed by their complex physiological structure and surface pigments. The system utilizes a deep learning model for body axis recognition and an adaptive Kalman filter to suppress abnormal visual feedback, achieving high-precision injection for zebrafish hearts.

REVIEW OF SCIENTIFIC INSTRUMENTS (2022)

Review Pharmacology & Pharmacy

Zebrafish as a tractable model of human cardiovascular disease

George Bowley et al.

Summary: The use of zebrafish models in studying cardiovascular development and disease mechanisms has become increasingly popular. By comparing the anatomy and physiology of zebrafish and mammalian cardiovascular systems, researchers have found that zebrafish have unique advantages in simulating cardiac and vascular pathologies.

BRITISH JOURNAL OF PHARMACOLOGY (2022)

Article Automation & Control Systems

DanioSense: Automated High-Throughput Quantification of Zebrafish Larvae Group Movement

Chunxiang Wang et al.

Summary: The study introduces DanioSense (DS), an automatic tracker for group larval zebrafish, that overcomes challenges such as similar appearances, frequent occlusions, and highly discontinuous kinematics through the integration of a light convolutional neural network and a centerline extraction algorithm. DS is capable of localizing individuals even in occlusion cases where object identities are prone to switch, providing detailed quantitative data for a large-scale larvae group in nearly real time.

IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING (2022)

Article Robotics

Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae

Ziqiang Chi et al.

Summary: This article introduces a new automated robotic system for batch microinjection of zebrafish larvae. The larvae are immobilized using a specially designed agarose supporting base, and injection targets are accurately identified using a machine vision scheme based on deep learning. Experimental results demonstrate that the robotic system provides higher injection success rate, better stability, and better performance than existing microinjection approaches.

IEEE ROBOTICS AND AUTOMATION LETTERS (2022)

Article Robotics

Visual-Guided Solutions in Automated Zebrafish Larva Heart Micro-Injection

Cheng Qian et al.

Summary: This study presents an automated micro-injection method for zebrafish larva heart, including key technologies such as auto-focusing, injection pipette tracking, and heart detection. By optimizing strategies and algorithms, the efficiency and accuracy of injection are improved, with a success rate of 90%.

IEEE ROBOTICS AND AUTOMATION LETTERS (2022)

Article Automation & Control Systems

Noncontact 3-D Orientation Control at Microscale: Hydrodynamic Out-of-Plane Rotation and In-Plane Rotation by Compacted Rotational Stage

Xiaoming Liu et al.

Summary: This article proposes a cost-effective solution for three-dimensional orientation control at the microscale, combining hydrodynamic rotation and piezo-actuator. Experimental results demonstrate the successful achievement of three-dimensional orientation control for microbeads and mouse oocytes.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2022)

Article Automation & Control Systems

7-DoFs Rotation-Thrust Microrobotic Control for Low-Invasive Cell Pierce via Impedance Compensation

Wanfeng Shang et al.

Summary: This article proposes a new robotic control method that reduces cell deformation and increases force stability during cell penetration by using rotation-thrust control and shear force compensation.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2022)

Article Robotics

Design and Development of a Teleoperated Robotic Microinjection System With Haptic Feedback

Kai Feng et al.

Summary: The proposed teleoperated robotic system for biological microinjection utilizes haptic feedback between the master and slave devices. Experimental results demonstrate the high resolution of the microforce sensor, fast response time of the haptic device, and faithful tracking of commands between the master and slave devices. The intuitive haptic feedback allows operators to better feel the microinjection process and improve success rate.

IEEE ROBOTICS AND AUTOMATION LETTERS (2021)

Article Computer Science, Artificial Intelligence

CNN-based visual processing approach for biological sample microinjection systems

Cheng Qian et al.

Summary: This study proposes an innovative convolutional neural network-based visual processing strategy for biological sample manipulation and microinjection systems. The approach allows for high-throughput injection with considerable injecting precision. Experimental results show significant improvement in orientation efficiency and enhanced accuracy of multi-sample rotation during the process of locating biological samples and rotating them.

NEUROCOMPUTING (2021)

Article Biochemistry & Molecular Biology

A novel strategy for glioblastoma treatment by induction of noptosis, an NQO1-dependent necrosis

Bingling Zhong et al.

Summary: In this study, tanshindiol B (TSB) inhibited the growth of glioblastoma by inducing a type of necrosis known as noptosis, which showed anti-tumor potential both in vitro and in vivo. TSB activated the NQO1 enzyme activity, leading to oxidative stress-induced necrotic cell death. These effects were mediated by c-Jun N-terminal kinase and completely abolished by a specific NQO1 inhibitor, dicoumarol.

FREE RADICAL BIOLOGY AND MEDICINE (2021)

Article Automation & Control Systems

Design and Testing of a New Force-Sensing Cell Microinjector Based on Small-Stiffness Compliant Mechanism

Yuzhang Wei et al.

Summary: Cell microinjection is crucial in the biomedical domain. A new force-sensing cell microinjector with novel compliant small-stiffness mechanism is proposed in this article, which enables high sensing sensitivity and large loading capability. The mechanism combines a constant-force mechanism with a small positive-stiffness mechanism to remove intercept force well.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2021)

Proceedings Paper Computer Science, Artificial Intelligence

Achieving Differential Privacy in Vertically Partitioned Multiparty Learning

Depeng Xu et al.

Summary: This paper proposes a new framework for preserving differential privacy in multiparty learning in a vertically partitioned setting, utilizing a functional mechanism by adding noise to the objective function. Evaluation on real-world and synthetic datasets for linear and logistic regressions shows the effectiveness of the proposed method.

2021 IEEE INTERNATIONAL CONFERENCE ON BIG DATA (BIG DATA) (2021)

Proceedings Paper Automation & Control Systems

Robotic Cardinal Vein Microinjection of Zebrafish Larvae Based on 3D Positioning

Mingzhu Su et al.

Summary: A robotic cardinal vein microinjection system was developed for zebrafish larvae, achieving a success rate of 85.0% and a survival rate of 94.1%. This system will greatly assist in tumor xenografts and cancer studies on zebrafish.

2021 IEEE INTERNATIONAL CONFERENCE ON ROBOTICS AND AUTOMATION (ICRA 2021) (2021)

Article Fisheries

Mitochondrial connections with immune system in Zebrafish

Mariana Abrantes do Amaral et al.

Summary: Mitochondria play multiple roles beyond ATP formation, including calcium homeostasis, apoptosis, autophagy, and ROS production. Changes in mitochondrial morphology rely on nutrient availability and bioenergetic demands, affecting cell fate decisions.

FISH AND SHELLFISH IMMUNOLOGY REPORTS (2021)

Review Chemistry, Medicinal

Use of Zebrafish in Drug Discovery Toxicology

Steven Cassar et al.

CHEMICAL RESEARCH IN TOXICOLOGY (2020)

Review Biochemistry & Molecular Biology

The Case for Modeling Human Infection in Zebrafish

Margarida C. Gomes et al.

TRENDS IN MICROBIOLOGY (2020)

Review Oncology

Zebrafish patient avatars in cancer biology and precision cancer therapy

Maurizio Fazio et al.

NATURE REVIEWS CANCER (2020)

Review Chemistry, Analytical

A Review of Automated Microinjection of Zebrafish Embryos

Yuliang Zhao et al.

MICROMACHINES (2019)

Article Engineering, Biomedical

Visual Servoed Three-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection System

Songlin Zhuang et al.

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING (2018)

Article Automation & Control Systems

Visual Servoed Zebrafish Larva Heart Microinjection System

Songlin Zhuang et al.

IEEE TRANSACTIONS ON INDUSTRIAL ELECTRONICS (2017)

Article Automation & Control Systems

Visual Detection and Two-Dimensional Rotation Control in Zebrafish Larva Heart Microinjection

Songlin Zhuang et al.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2017)

Article Developmental Biology

Microstructured Surface Arrays for Injection of Zebrafish Larvae

Felix Ellett et al.

ZEBRAFISH (2017)

Article Computer Science, Information Systems

Haptic Microrobotic Cell Injection System

Ali Ghanbari et al.

IEEE SYSTEMS JOURNAL (2014)

Article Biophysics

Vision-based force measurement using neural networks for biological cell microinjection

Fatemeh Karimirad et al.

JOURNAL OF BIOMECHANICS (2014)

Article Developmental Biology

Low-Cost Silicone Imaging Casts for Zebrafish Embryos and Larvae

Wouter Masselink et al.

ZEBRAFISH (2014)

Article Automation & Control Systems

Force Sensing and Manipulation Strategy in Robot-Assisted Microinjection on Zebrafish Embryos

Yu Xie et al.

IEEE-ASME TRANSACTIONS ON MECHATRONICS (2011)

Article Engineering, Electrical & Electronic

A micromanipulation system with dynamic force-feedback for automatic batch microinjection

Zhe Lu et al.

JOURNAL OF MICROMECHANICS AND MICROENGINEERING (2007)