Journal
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
Volume 13, Issue 12, Pages 2319-2323Publisher
SPRINGER
DOI: 10.1007/s13738-016-0951-6
Keywords
Ketoprofen; Chiral separation; Vancomycin; Chiral mobile phase additive
Categories
Ask authors/readers for more resources
A high-performance liquid chromatographic method for chiral separation of ketoprofen racemate was developed. (R)- and (S)-ketoprofen enantiomers were separated on a LiChrosorb NH2 column (250 mm x 4.6 mm, i.d 5 A mu m) at 20 A degrees C, using 2-propanol/potassium dihydrogen phosphate buffer (pH 6.0, 0.05 M) (50:50 v/v). Containing vancomycin as the mobile phase, at a flow rate of 0.8 ml min(-1) and detection wavelength of UV, the detector was set at 310 nm. Under these conditions, ketoprofen enantiomers could be separated with a selectivity factor (alpha) of 2.172 and a resolution (Rs) of 4.78 using extremely low concentrations of the vancomycin chiral additive.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available