Journal
FUTURE MICROBIOLOGY
Volume 18, Issue 9, Pages 563-580Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2022-0238
Keywords
EMSA; gene expression; MSMEG_5850; Mycobacterium; TetR-like transcription factors; transcriptome analysis
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This study elucidated the role of MSMEG_5850 in the physiology of mycobacteria. Transcriptome analysis revealed differential expression of 148 genes in an MSMEG_5850 knockout strain. Electrophoretic mobility shift assay and size exclusion chromatography confirmed the binding of MSMEG_5850 to its motif as a monomer. MSMEG_5850 was upregulated under nutritional stress and promoted the survival of mycobacteria.
Aim: To decipher the role of MSMEG_5850 in the physiology of mycobacteria. Methods: MSMEG_5850 was knocked out and RNA sequencing was performed. MSMEG_5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG_5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG_5850 knockout strain. MSMEG_5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG_5850 bound to its motif as a monomer. MSMEG_5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG_5850 in global transcriptional regulation.
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