Genomic insights into extensively drug-resistant Pseudomonas aeruginosa isolated from a diarrhea case in Kolkata, India

Aim: To characterize extensively drug-resistant Pseudomonas aeruginosa from a patient with diarrhea. Materials & methods: Antimicrobial susceptibility was tested by the disk diffusion method. The P. aeruginosa genome was sequenced to identify virulence, antibiotic resistance and prophages encoding genes. Results: P. aeruginosa had a wide spectrum of resistance to antibiotics. Genomic analysis of P. aeruginosa revealed 76 genes associated with antimicrobial resistance, xenobiotic degradation and the type three secretion system. Conclusion: This is the first report on diarrhea associated with P. aeruginosa. Since no other organism was identified, the authors assume that the patient had dysbiosis due to antibiotic exposure, leading to antibiotic-associated diarrhea. The in vivo toxicity expressed by the pathogen may be associated with T3SS.

Automated summary

The aim of this study was to characterize extensively drug-resistant Pseudomonas aeruginosa from a patient with diarrhea. Antimicrobial susceptibility testing revealed that P. aeruginosa had a wide spectrum of resistance to antibiotics. Genomic analysis identified 76 genes associated with antimicrobial resistance, xenobiotic degradation, and the type three secretion system. This is the first report on diarrhea associated with P. aeruginosa, suggesting dysbiosis due to antibiotic exposure and the potential role of T3SS in in vivo toxicity of the pathogen.