Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume -, Issue -, Pages -Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-023-01316-y
Keywords
Piaractus brachypomus fish meat; Fermentative hydrolysis; Antioxidant activity; Peptide purification; Peptide identification
Categories
Ask authors/readers for more resources
The study focused on the preparation, purification, and identification of novel antioxidant peptides from Piaractus brachypomus fish meat. The RPMPH-IF fraction, obtained by ultrafiltration, displayed significantly higher antioxidant activities. After purification by gel filtration and reversed-phase liquid chromatography, the RPH-8 fraction showed the highest antioxidant activities. The peptide sequences of RPH-8 fraction were identified using LC-MS/MS and MASCOT software.
The current study investigates the preparation, purification, and identification of novel antioxidant peptides from Piaractus brachypomus fish (RBPF) meat. Antioxidant peptides from RBPF meat protein hydrolysate (RPMPH) were fractionated by ultrafiltration (3 kDa MWCO membrane). RPMPH-IF (MW < 3 kDa) fraction displayed significantly higher antioxidant activities (P < 0.05) (DPPH, ABTS, FRAP, and Fe(2+)chelating activity). RPMPH-IF was purified by Sephadex G-25 gel filtration chromatography, and the RPMPH-1 fraction exhibited significantly higher antioxidant activities (P < 0.05). Subsequently, the RPMPH-1 fraction was purified by reversed-phase high-performance liquid chromatography. RPH-8 showed the highest antioxidant activities. The sequence of peptides of the RPH-8 fraction was later identified by LC-MS/MS and MASCOT software. RPH-8 fraction showed the two peptides with MW of 1105.52 Da and 748.25 Da, and the sequence of peptides was identified as His-Asn-Leu-Gly-Leu-Leu-His-Gly-Asp-Met and Asp-Ala-Pro-Ser-Met-Asn-Asp, respectively. Thus, RPMPH or purified antioxidant peptides produced by probiotic Bacillus strain could be a bio-functional ingredient in food and nutraceutical applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available