Infection behavior of Listeria monocytogenes on iceberg lettuce (Lactuca sativa var. capitata)

Iceberg lettuce among leafy vegetables is susceptible to contamination with foodborne pathogens, posing a risk of food microbial safety. Listeria monocytogenes (L. monocytogenes) is a highly lethal pathogen that can survive and proliferate on leafy vegetables. In this paper, the contamination stage, attachment site, internalization pathway, proliferation process, extracellular substance secretion and virulence factors expression of L. monocytogenes on iceberg lettuce were researched. Results showed that the contamination stage of L. monocytogenes on iceberg lettuce was 0-20 min, the proliferation stage was after 20 min. The attachment tissues were stomata and winkles. The internalization distance of L. monocytogenes in the midrib was farther than that in the leaf blade. They enhanced the movement ability of cells by up-regulating the expression of flaA and motA genes, and enhanced the adhesion ability of cells by up-regulating the expression of actA and inla genes, which was beneficial to the proliferation. During proliferation, cells gradually secreted extracellular substances to promote the biofilm formation on iceberg lettuce. The formation of biofilms experienced: individual bacteria, cell aggregation and biofilm maturation. Biofilms were more likely to form on the leaf blade of iceberg lettuce.

Automated summary

This study investigated the contamination stage, attachment site, internalization pathway, proliferation process, extracellular substance secretion, and virulence factors expression of L. monocytogenes on iceberg lettuce. The results showed that the contamination stage occurred within 0-20 minutes, followed by proliferation. Attachment was observed in stomata and winkles. L. monocytogenes showed greater internalization distance in the midrib compared to the leaf blade. The up-regulation of flaA and motA genes enhanced cell movement, while the up-regulation of actA and inla genes enhanced adhesion, favoring proliferation. Cells gradually secreted extracellular substances to form biofilms, with a preference for the leaf blade.