Journal
FOOD RESEARCH INTERNATIONAL
Volume 168, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2023.112744
Keywords
Procyanidin; ?-lactoglobulin; Glyco-oxidation; Free radicals; Glycation sites
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In this study, the effects and underlying mechanisms of B type procyanidin (PC) on milk protein beta-lactoglobulin (BLG) glyco-oxidation induced by free radicals and metal ions were investigated. The results showed that PC protected BLG from structural changes caused by cross-linking and aggregation. Additionally, PC inhibited BLG oxidation and glycation, reducing carbonyls and advanced glycation end-products (AGEs). PC exhibited free radical scavenging and metal chelating properties, and it also formed procyanidin-MGO conjugates to interrupt BLG glycation. These findings suggest that B type procyanidin is an effective glyco-oxidation inhibitor in milk products.
Procyanidin is a group of dietary flavonoids abundant in berry fruits. In this study, the effects and underlying mechanisms of B type procyanidin (PC) on free radical and metal ion (H2O2, AAPH and Fe3+) induced milk protein beta-lactoglobulin (BLG) glyco-oxidation were investigated. The results indicated that PC protected BLG structure changes from cross-link and aggregation induced by free radicals and metal ion. Additionally, it effectively inhibited BLG oxidation by reducing approximately 21%-30% carbonyls and 15%-61% schiff base crosslink formation. Also, PC suppressed BLG glycation by inhibiting 48-70% advanced glycation end-products (AGEs) and reduced the accumulation of intermediate product methylglyoxal (MGO). The corresponding mechanisms were elucidated that PC exhibited great free radical scavenging and metal chelating properties; PC had non-covalent bind with the amino acid residues (preferably lysine and arginine) of BLG and blocked them from glycation; PC interrupted BLG glycation by forming procyanidin-MGO conjugates. Therefore, B type pro-cyanidin was an effective glyco-oxidation inhibitor in milk products.
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