Microrheological properties and local structure of ι-carrageenan gels probed by using optical tweezers

Iota carrageenan (IC) is one of the most important gelling carrageenans in food and biotechnological applica-tions; however, some of its potential applications have been constrained by its weak gelation ability. In order to better understand the origin of the weak mechanical response of IC gels, the hierarchical network structure based on meso-and microrheological properties of IC gels at 20 degrees C were probed via passive and active microrheology both performed by using optical tweezers (OT). Passive microrheology captures a wide spectral content of IC viscoelastic properties, revealing a rubbery plateau of the elastic modulus at relatively high frequencies for all IC concentrations; thus, uncovering the dynamics of the network. Moreover, different microstructures of IC gels were inferred by analyzing the concentration-dependent stress response when applying large deformation to the network. At low IC concentration, yielding was observed as indicated by the stress-independent response vs. strain, implying the structural rearrangement and disentanglement. As the IC concentration increased, the yielding diminished with increasing strain rate due to increased entanglement density, which limits the rear-rangement of clusters. Therefore, this study presents novel insights into the meso-and microscale properties of IC gels that otherwise would not be accessible by conventional bulk rheology and submicroscopic probe diffusion measurements.

Automated summary

In this study, the hierarchical network structure of Iota carrageenan (IC) gels was investigated using passive and active microrheology performed by optical tweezers at 20 degrees C. The results showed that IC gels have a rubbery plateau of the elastic modulus, and the dynamics of the network decrease with increasing cluster density. This study provides novel insights into the meso- and microscale properties of IC gels that are not accessible by conventional bulk rheology and submicroscopic probe diffusion measurements.