Dual-template hydrophilic imprinted resin as an adsorbent for highly selective simultaneous extraction and determination of multiple trace plant growth regulators in red wine samples

In recent years, numerous plant growth regulators have been found in foods and have a toxicity to human health, so its simultaneous multiple monitoring is urgently. For the first time, a rapid, accurate, and high-selective method was established to extract and determine multiple plant growth regulators simultaneously in red wines using a new dual-template hydrophilic molecularly imprinted resin (DHMIR) as an adsorbent of pipette tip solid-phase extraction coupled with HPLC. The as-prepared DHMIR combined the advantages of the hydrophi-licity of hydrophilic resin and multi-imprinted recognition of dual-template molecular imprinting, overcoming the poor imprinted recognition ability of traditional imprinting materials in water and low extraction efficiency to multiple targets. Under the optimized conditions, the proposed method exhibited high sensitivity (2.29-3.94 ng mL-1) and recoveries (80.9-109.0 %) using only 15 mg DHMIR. This study provides an effective strategy for rapid, accurate, low-cost, and high-selective determination of the multiple analytes in food samples.

Automated summary

In recent years, the presence of multiple plant growth regulators in foods and their toxicity to human health has raised the need for simultaneous multiple monitoring. A new method utilizing a dual-template hydrophilic molecularly imprinted resin (DHMIR) coupled with HPLC has been established, allowing for rapid, accurate, and high-selective determination of multiple plant growth regulators in red wines. The DHMIR combines the advantages of hydrophilic resin and dual-template molecular imprinting, overcoming the shortcomings of traditional imprinting materials in water and low efficiency in extracting multiple targets. Under optimized conditions, the proposed method demonstrates high sensitivity (2.29-3.94 ng/mL) and recoveries (80.9-109.0%) using only 15 mg DHMIR. This study provides an effective strategy for the determination of multiple analytes in food samples that is rapid, accurate, low-cost, and high-selective.