Stereoselective analysis of chiral succinate dehydrogenase inhibitors (SDHIs) in foods of plant origin and animal origin by supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS)

The stereoisomers of chiral SDHIs were prepared using Autoprep HPLC and chiral columns. The method of combining theoretical calculation with experimental determination was used to confirm the absolute configuration of stereoisomer. SFC-MS/MS and four kinds of chiral columns were used to separate the eight chiral SDHIs, and they could be separated simultaneously using OD-3 column in 6.5 min. The integrated QuEChERS strategy was used to analyse the chiral SDHIs in foods of plant and animal origin, and the average recoveries ranged from 71 % to 119 % with RSD <= 18 %, and the LOQ was 1 ng/g. There were 99.2 % and 63.6 % matrix effects were in the range of 0.8-1.2 in foods of plant and animal origin, respectively, showing weak matrix effects. The study provided methods for monitoring chiral SDHIs stereoisomers residues, which were crucial for stereoselective evaluations and improving risk assessments.

Automated summary

The stereoisomers of chiral SDHIs were prepared and their absolute configuration was confirmed using Autoprep HPLC and chiral columns combined with theoretical calculation and experimental determination. SFC-MS/MS and four types of chiral columns were used to separate the chiral SDHIs, with simultaneous separation achieved in 6.5 minutes using the OD-3 column. The QuEChERS strategy was used to analyze the chiral SDHIs in plant and animal food samples, with average recoveries ranging from 71% to 119% and RSD <= 18%, and the LOQ was 1 ng/g. The matrix effects in plant and animal food samples ranged from 0.8 to 1.2, with weak matrix effects observed. This study provides important methods for monitoring the residues of chiral SDHI stereoisomers, which are crucial for stereoselective evaluations and improving risk assessments.