4.7 Article

Glycation with uronic acid-type reducing sugar enhances the anti-inflammatory activity of fish myofibrillar protein via the Maillard reaction

Journal

FOOD CHEMISTRY
Volume 407, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.135162

Keywords

Fish myofibrillar protein; Anti-inflammatory activity; Glycation; Maillard reaction; Uronic acid; Carboxyl group

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This study investigated the role of carboxyl group in uronic acid in enhancing the anti-inflammatory activity of fish myofibrillar protein. Lyophilized Mf was reacted with various reducing sugars through the Maillard reaction, and the anti-inflammatory activity was evaluated. The results showed that glycation with uronic acid-type reducing sugars greatly enhanced the anti-inflammatory activity of Mf, while glycation with glucose or galactose had no significant effect. The study also suggested that the number of carboxyl group in bound reducing sugar could be a determining factor for the enhanced effect.
The role of carboxyl group in uronic acid in enhancing the anti-inflammatory activity of fish myofibrillar protein (Mf) was investigated, when lyophilized Mf was reacted with various reducing sugars at 60 degrees C and 35% relative humidity through the Maillard reaction. After pepsin and trypsin digestion, the anti-inflammatory activity was evaluated by measuring the secretions of tumor necrosis factor-alpha, interleukin-6, interleukin-10, and nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophage. The anti-inflammatory activity of Mf was not affected by glycation with glucose or galactose, whereas strongly enhanced by glycation with uronic acid-type reducing sugars: glucuronic acid, galacturonic acid, and alginate oligosaccharide. These results indicate that the presence of carboxyl group in reducing sugar is important for enhancing the anti-inflammatory activity of Mf. This study also shows that the enhanced effect could depend upon the number of carboxyl group in bound reducing sugar.

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