☆ 4.5 Article

High-level itaconic acid (IA) production using engineered Escherichia coli Lemo21(DE3) toward sustainable biorefinery

ENZYME AND MICROBIAL TECHNOLOGY (2023)

Related references

Note: Only part of the references are listed.

Article Biochemistry & Molecular Biology

A molecular device for the redox quality control of GroEL/ES substrates

Emile Dupuy et al.

Summary: Hsp60 chaperonins and their Hsp10 cofactors facilitate protein folding. This study reveals that the bacterial chaperonin GroEL forms a stable complex with the chaperedoxin CnoX, and CnoX likely functions as a redox quality-control plugin for GroEL. The findings suggest that Hsp60 molecular plugins have been conserved through evolution, as proteins sharing structural features with CnoX exist in eukaryotes.

CELL (2023)

Add to Collection

Article Biochemistry & Molecular Biology

Genetic design of co-expressing a novel aconitase with cis-aconitate decarboxylase and chaperone GroELS for high-level itaconic acid production

Chuan-Chieh Hsiang et al.

Summary: In this study, a new aconitase from acidophilic E. coli Nissle 1917, denoted as ENAcnA, was compared to Corynebacterium glutamicum for the first time. Co-expression of ENAcnA and cis-aconitate decarboxylase from Aspergillus terreus in recom-binant E. coli BL21(DE3) resulted in high-level IA production. Integration of GroELS chaperon into the chromosome of BL21(DE3) and cold treatment of the whole-cell biocatalyst further enhanced IA production.

PROCESS BIOCHEMISTRY (2023)

Add to Collection

Article Biotechnology & Applied Microbiology

Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli

Shih-Chen Yang et al.

Summary: The demand for bio-based materials to replace traditional plastics is growing. In this study, a time-efficient method was developed to convert L-arginine into putrescine using whole cell bioconversion. It was found that the key enzymes from different combinations of plasmids can effectively collaborate, achieving a high conversion rate.

BIOCHEMICAL ENGINEERING JOURNAL (2022)

Add to Collection

Article Agricultural Engineering

Acetate-based production of itaconic acid with Corynebacterium glutamicum using an integrated pH-coupled feeding control

Manuel Merkel et al.

Summary: To date, most bio-based products of industrial biotechnology are derived from sugar-based carbon sources, which compete with food and feed resources. In this study, an engineered strain of Corynebacterium glutamicum ATCC 13032 was used to produce itaconic acid from acetate, overcoming current production challenges. The results show the potential of using acetate as an alternative substrate for bioproduction, with a high peak volumetric productivity and itaconate titer achieved.

BIORESOURCE TECHNOLOGY (2022)

Add to Collection

Article Biotechnology & Applied Microbiology

Tailoring key enzymes for renewable and high-level itaconic acid production using genetic Escherichia coli via whole-cell bioconversion

Chuan-Chieh Hsiang et al.

Summary: Renewable chemical production through carbon-neutral design has received significant attention. In this study, the WC bioconversion of citrate into IA using key enzymes ACN and CAD showed promising results, with CAD identified as the rate-determining step. Metal ions like copper and ferric ions were found to inhibit enzyme activity when both enzymes co-worked. The engineered E. coli expressing dual genes achieved a high IA titer and productivity, demonstrating a promising renewable process.

ENZYME AND MICROBIAL TECHNOLOGY (2022)

Add to Collection

Article Biochemical Research Methods

Reprogramming T7RNA Polymerase in Escherichia coli Nissle 1917 under Specific Lac Operon for Efficient p-Coumaric Acid Production

Sefli Sri Wahyu Effendi et al.

Summary: In this study, the impact of Lac operon on protein expression in the T7-derived EcN strains was investigated. Through fine-tuning different parameters, the conversion rate of p-coumaric acid, a bioactive compound beneficial for human health, was significantly improved in simulated gut conditions. These findings highlight the potential of using EcN as an efficient cell factory for live bacterial therapeutics.

ACS SYNTHETIC BIOLOGY (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Genetic design of co-expressed Mesorhizobium loti carbonic anhydrase and chaperone GroELS to enhancing carbon dioxide sequestration

Sefli Sri Wahyu Effendi et al.

Summary: The study found that co-expressing MlCA with protein partners effectively increased the solubility, activity, and turnover rate, while also affecting its stability in temperature, pH range, and presence of metal ions.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Migration of glutamate decarboxylase by cold treatment on whole-cell biocatalyst triggered activity for 4-aminobutyric acid production in engineering Escherichia coli

Chengfeng Xue et al.

Summary: By overexpressing GadB in Escherichia coli and subjecting it to cold treatment, the enzymatic turnover rate and migration rate of GadB increased, leading to improved GABA production in whole-cell biotransformation.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection

Article Materials Science, Multidisciplinary

Surface Functionalization Utilizing Mesoporous Silica Nanoparticles for Enhanced Evanescent-Field Mid-Infrared Waveguide Gas Sensing

Diana Al Husseini et al.

Summary: This work focuses on developing nanoparticle-based layer-by-layer coatings on-chip mid-infrared waveguides to enhance the detection sensitivity and selectivity of volatile organic compounds. The study shows that the improved sensing performance is achieved through preferential adsorption of specific molecules within the nanoparticles near the waveguide evanescent field.

COATINGS (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Development of Escherichia coli Nissle 1917 derivative by CRISPR/Cas9 and application for gamma-aminobutyric acid (GABA) production in antibiotic-free system

Yi-Jun Lan et al.

Summary: This study successfully employed CRISPR/Cas9-based plasmid curing to construct a plasmid-free E. coli EcNP and developed stable expression vectors pMT1 and pMT2. These strategies enhanced the applicability of EcN for potential genetic engineering applications.

BIOCHEMICAL ENGINEERING JOURNAL (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110

Wan-Wen Ting et al.

Summary: This study established a stable and high-level expression system for CadA production, achieving high productivity and yield of cadaverine in engineered E. coli strain JW. The whole-cell biocatalyst showed excellent reusability and potential for bio-nylon production in the future.

ENZYME AND MICROBIAL TECHNOLOGY (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Overcoming glutamate auxotrophy in Escherichia coli itaconate overproducer by the Weimberg pathway

Ken W. Lu et al.

Summary: Engineering efficient itaconate producers often requires elimination of isocitrate dehydrogenase to preserve cis-aconitate, leading to auxotrophy for 2-ketoglutarate. This study presents a novel approach by introducing the Weimberg pathway for 2-ketoglutarate biosynthesis, overcoming the need for glutamate supplementation. Through careful tuning of xylose concentration, the final strain produced 20 g/L of itaconate in minimal medium without the need for antibiotics.

METABOLIC ENGINEERING COMMUNICATIONS (2021)

Add to Collection

Article Biotechnology & Applied Microbiology

Accelerating itaconic acid production by increasing membrane permeability of whole-cell biocatalyst based on a psychrophilic bacterium Shewanella livingstonensis Ac10

Gonglinfeng Luo et al.

JOURNAL OF BIOTECHNOLOGY (2020)

Add to Collection

Article Chemistry, Physical

Machine Learning in Enzyme Engineering

Stanislav Mazurenko et al.

ACS CATALYSIS (2020)

Add to Collection

Article Agriculture, Multidisciplinary

A Strategy to Improve Production of Recombinant Proteins in Escherichia coli Based on a Glucose-Glycerol Mixture and Glutamate

Chung-Jen Chiang et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

Consolidated bioprocessing of cellulose to itaconic acid by a co-culture of Trichoderma reesei and Ustilago maydis

Ivan Schlembach et al.

BIOTECHNOLOGY FOR BIOFUELS (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

Carbon catabolite repression relaxation in Escherichia coli: global and sugar-specific methods for glucose and secondary sugar co-utilization

Kevin J. Fox et al.

CURRENT OPINION IN CHEMICAL ENGINEERING (2020)

Add to Collection

Review Chemistry, Multidisciplinary

Enzymatic Cascade Reactions in Biosynthesis

Christopher T. Walsh et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

Add to Collection

Article Multidisciplinary Sciences

Enhancement of biocatalyst activity and protection against stressors using a microbial exoskeleton

Jonathan K. Sakkos et al.

SCIENTIFIC REPORTS (2019)

Add to Collection

Article Biochemistry & Molecular Biology

Enhanced production of poly-3-hydroxybutyrate (PHB) by expression of response regulator DR1558 in recombinant Escherichia coli

Sung-ho Park et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Add to Collection

Article Multidisciplinary Sciences

Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance

Tanita Wein et al.

NATURE COMMUNICATIONS (2019)

Add to Collection

Article Biotechnology & Applied Microbiology

Integrated strain- and process design enable production of 220 g L-1 itaconic acid with Ustilago maydis

Hamed Hosseinpour Tehrani et al.

BIOTECHNOLOGY FOR BIOFUELS (2019)

Add to Collection

Article Biotechnology & Applied Microbiology

Enhanced itaconic acid production in Yarrowia lipolytica via heterologous expression of a mitochondrial transporter MTT

Chen Zhao et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2019)

Add to Collection

Review Biochemistry & Molecular Biology

Biocatalytic strategies in the production of galacto-oligosaccharides and its global status

Parmjit S. Panesar et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Strategies for optimization of heterologous protein expression in E-coli: Roadblocks and reinforcements

Jashandeep Kaur et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

Add to Collection

Article Multidisciplinary Sciences

A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins

Federica Angius et al.

SCIENTIFIC REPORTS (2018)

Add to Collection

Article Biotechnology & Applied Microbiology

Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli

Zhongwei Yang et al.

BIOTECHNOLOGY AND BIOENGINEERING (2017)

Add to Collection

Review Biotechnology & Applied Microbiology

Whole cell biocatalysts: essential workers from Nature to the industry

Carla C. C. R. de Carvalho

MICROBIAL BIOTECHNOLOGY (2017)

Add to Collection

Review Biotechnology & Applied Microbiology

Whole-cell biocatalysts by design

Baixue Lin et al.

MICROBIAL CELL FACTORIES (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Itaconic acid production from wheat chaff by Aspergillus terreus

Susan Krull et al.

PROCESS BIOCHEMISTRY (2017)

Add to Collection

Article Biotechnology & Applied Microbiology

Modulation of heterologous expression from PBAD promoter in Escherichia coli production strains

Diana Szeliova et al.

JOURNAL OF BIOTECHNOLOGY (2016)

Add to Collection

Review Biochemistry & Molecular Biology

The GroEL-GroES Chaperonin Machine: A Nano-Cage for Protein Folding

Manajit Hayer-Hartl et al.

TRENDS IN BIOCHEMICAL SCIENCES (2016)

Add to Collection

Article Multidisciplinary Sciences

Identification of protein secretion systems in bacterial genomes

Sophie S. Abby et al.

SCIENTIFIC REPORTS (2016)

Add to Collection

Article Biochemistry & Molecular Biology

Cold Stress Makes Escherichia coli Susceptible to Glycopeptide Antibiotics by Altering Outer Membrane Integrity

Jonathan M. Stokes et al.

Cell Chemical Biology (2016)

Add to Collection

Article Biotechnology & Applied Microbiology

Rich Medium Composition Affects Escherichia coli Survival, Glycation, and Mutation Frequency during Long-Term Batch Culture

Karin E. Kram et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

Metabolic engineering of Corynebacterium glutamicum for the production of itaconate

Andreas Otten et al.

METABOLIC ENGINEERING (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

Metabolic engineering of Yarrowia lipolytica for itaconic acid production

John Blazeck et al.

METABOLIC ENGINEERING (2015)

Add to Collection

Review Biochemistry & Molecular Biology

Understanding carbon catabolite repression in Escherichia coli using quantitative models

A. Kremling et al.

TRENDS IN MICROBIOLOGY (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

Metabolic engineering of itaconate production in Escherichia coli

Kiira S. Vuoristo et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

High-level production of membrane proteins in E-coli BL21(DE3) by omitting the inducer IPTG

Zhe Zhang et al.

MICROBIAL CELL FACTORIES (2015)

Add to Collection

Article Biotechnology & Applied Microbiology

Influence of the pH on the itaconic acid production with Aspergillus terreus

Antje Hevekerl et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

Add to Collection

Review Biotechnology & Applied Microbiology

The transport and mediation mechanisms of the common sugars in Escherichia coli

Yane Luo et al.

BIOTECHNOLOGY ADVANCES (2014)

Add to Collection

Article Biotechnology & Applied Microbiology

Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications

Ashty S. Karim et al.

FEMS YEAST RESEARCH (2013)

Add to Collection

Article Biochemistry & Molecular Biology

GroEL/ES Buffering and Compensatory Mutations Promote Protein Evolution by Stabilizing Folding Intermediates

Kirsten T. Wyganowski et al.

JOURNAL OF MOLECULAR BIOLOGY (2013)

Add to Collection

Article Biotechnology & Applied Microbiology

Targeting enzymes to the right compartment: Metabolic engineering for itaconic acid production by Aspergillus niger

Marzena L. Blumhoff et al.

METABOLIC ENGINEERING (2013)

Add to Collection

Editorial Material Biotechnology & Applied Microbiology

Biobased chemicals: the convergence of green chemistry with industrial biotechnology

Jim C. Philp et al.

TRENDS IN BIOTECHNOLOGY (2013)

Add to Collection

Review Biotechnology & Applied Microbiology

Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus

Mitsuyasu Okabe et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2009)

Add to Collection

Article Biochemistry & Molecular Biology

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

Bryson D. Bennett et al.

NATURE CHEMICAL BIOLOGY (2009)

Add to Collection

Article Biotechnology & Applied Microbiology

Cloning and functional characterization of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus

Shin Kanamasa et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2008)

Add to Collection

Article Biochemical Research Methods

Consequences of membrane protein overexpression in Escherichia coli

Samuel Wagner et al.

MOLECULAR & CELLULAR PROTEOMICS (2007)

Add to Collection

© Peeref 2019-2024. All rights reserved.