Silk sericin conjugated magnesium oxide nanoparticles for its antioxidant, anti-aging, and anti-biofilm activities

The Silk sericin protein was conjugated with magnesium oxide (MgO) nanoparticles to form SS-MgO-NPs . UV, XRD, FTIR, SEM, DLS, and EDX were used to confirm the formation of SS-MgO-NPs. The absorption band of SS-MgO-NPs using UV-visible spectra was observed at 310 nm, with an average size of the nanoparticles was 65-88 nm analyzed from DLS. The presence of alcohol, C--N, and C--C, alkanes, alkenes, and cis alkenes, in silk sericin, is confirmed by FT-IR and may act as a stabilizing agent. Later SS-MgO-NPs were evaluated for antioxidant, antibacterial, anti-biofilm, ,anti-aging, and anticancer properties. The SS-MgO-NPs inhibited the formation of biofilm of Pseudomonas aeruginosa and Bacillus cereus. The blood compatibility of SS-MgO-NPs, delaying coag-ulation was observed using human, blood, and goat blood samples. The SS-MgO-NPs exhibited significant anticancer activity on MCF-7 (IC50 207.6 mu g/mL) cancer cell lines. Correspondingly, SS-MgO-NPs demonstrated dose-dependent inhibition of the enzymes in the following order collagenase > elastase > tyrosinase > hyal-uronidase, with IC50 values of 75.3, 85.3, 133.6, and 156.3 mu gmL-1, respectively. This exhibits the com-poundposses anti-aging properties. So, in in vitro settings, SS-MgO-NPs can be used as an antibacterial, anti-aging, and anticancer agent. Additionally, in vivo research is necessary to validate its therapeutic applications.

Automated summary

The study focused on the conjugation of silk sericin protein with magnesium oxide nanoparticles to form SS-MgO-NPs. Various techniques, such as UV, XRD, FTIR, SEM, DLS, and EDX, were used to confirm the formation of SS-MgO-NPs. The SS-MgO-NPs showed promising properties, including antioxidant, antibacterial, anti-biofilm, anti-aging, and anticancer activities. Further in vivo research is necessary to validate the therapeutic applications of SS-MgO-NPs.