Simultaneous determination of heavy metal concentrations and toxicities by diffusive gradient in thin films containing Acinetobacter whole-cell bioreporters (Bio-DGT)

Heavy metal contaminations may cause severe toxic impacts to ecological systems and human health. Mea-surements of metals' bioavailable concentrations and toxicities simultaneously and in-situ in environments can advance the understanding of their hazardous effects. The diffusive gradients in thin-films (DGT) is an in-situ technique can measure metal speciation and labile concentrations, but cannot yet provide the direct toxicity information. The whole-cell bioreporter Acinetobacter baylyi ADPWH_recA was successfully incorporated into the DGT device to develop a novel technique, Bio-DGT, for assessing the toxicity of metals at the same time of measuring their labile concentrations. The bioassay used in Bio-DGT can sense the mixture toxicity from multiple contaminants and the DGT can assist in identifying which toxicants may be causing the toxicity. Cadmium was used as the model metal to test the performance of Bio-DGT in waters and soils. The masses of Cd accumulated in Bio-DGT increased linearly and theoretically with time for 7 days deployment, indicating little influences from bioreporter cells on DGT performance. A positive relationship between bioluminescent signals towards Cd demonstrated the sensitive and active bioreporters' response. The sensitive of Bio-DGT, indicated by Cd con-centrations causing the response, is 0.01 mg/L. The stable response from Bio-DGT under various conditions (pH 4-8, ionic strengths 0.01-0.5 M) and 30 days storage time suggest the applicability of the technique in real environments. The deployment of Bio-DGT in contaminated soils demonstrated that Cd toxicity was regulated by labile concentration, showing its potential application for the risk assessment of heavy metal contaminations, and its further feasibility in using Bio-DGT for measuring integration of multiple contaminants' effects and simul-taneously determine the main toxicity driver(s).

Automated summary

Heavy metal contaminations can have severe toxic impacts on ecological systems and human health. This study introduces a novel technique called Bio-DGT, which combines the use of a bioassay with the diffusive gradients in thin-films (DGT) method to simultaneously assess the toxicity of metals while measuring their labile concentrations. The results show that Bio-DGT has good sensitivity and stability, making it a potential tool for risk assessment of heavy metal contaminations.