Influence of ionic cerium and cerium oxide nanoparticles on Zea mays seedlings grown with and without cadmium

Cerium (Ce4+) and cerium oxide nanoparticles (CeO2-NPs) have diversified reported effects on plants. Once dispersed in the environment their fate is not well understood, especially in co-existence with other pollutants like cadmium (Cd). The effect of co-application of Ce and Cd are reported in various studies, but the role of Ce source (ionic or bulk) and nanoparticle size is still unknown in cereal plants like maize (Zea mays). To better understand the synergistic effects of Ce and Cd, 500 mg kg(-1) Ce coming from ionic (Ce4+ as CeSO4) and CeO2 nano sources (10 nm, 50 nm, and 100 nm) alone and in combination with 0.5 mg Cd kg(-1) sand were applied to maize seedlings. Growth, physiology, root structure, anatomy, and ionic homeostasis in maize were measured. The results revealed that Ce4+ resulted in overall decrease in seedling growth, biomass and resulted in higher heavy metal (in control sets) and Cd (in Cd spiked sets) uptake in maize seedlings' root and shoot. The effects of CeO2-NPs were found to be dependent on particle size; in fact, under Cd-0 (non-Cd spiked sets) CeO2-100 nm showed beneficial effects compared to the control. While under co-application with Cd, CeO2-50 nm showed net beneficial effects on maize seedling growth parameters. The Ce alone, and in combination with Cd, altered the root suberin barrier formation. Both ionic and nano Ce sources alone and in co-existence with Cd behaved differently for tissue elemental concentrations (Ce, Cd, micronutrients like B, Mn, Ni, Cu, Zn, Mo, Fe and elements Co, Si) suggesting a strong influence of Cd-Ce coexistence on the element's uptake and translocation in maize.

Automated summary

The effects of cerium (Ce4+) and cerium oxide nanoparticles (CeO2-NPs) on maize plants were studied, and the role of Ce source (ionic or bulk) and nanoparticle size in maize was investigated. It was found that Ce4+ resulted in decreased seedling growth and biomass, and increased uptake of heavy metals and Cd in maize. The effects of CeO2-NPs depended on particle size, with CeO2-50 nm showing beneficial effects on maize seedling growth parameters in the presence of Cd.