Amperometric detection of indapamide on glassy carbon electrode

This work describes the development of a fast, precise and reliable voltammetric method for the quantification of indapamide, an orally active diuretic sulfonamide used for hypertensive treatment. This compound acts inhibiting sodium reabsorption and increasing the elimination of water. This characteristic was responsible for its banishment by the International Olympic Committee since 1999. The study begins by finding an adequate potential range (-0.20 to 0.30 V) to avoid poisoning the working glassy carbon electrode (GCE) in phosphate buffer 0.10 mol L-1 (pH=12.0). Utilizing flow injection analysis, linear responses between 2.0 x 10(-6) mol L-1 to 2.5 x 10(-5) mol L-1 of indapamide (R-2=0.995), and detection limit (LOD) 3.0 x 10(-7) mol L-1 were obtained. This method was applied for the quantification of indapamide in tablets and in synthetic urine. The same flow system was used for the analysis of commercial drugs and the response obtained corresponded to 98 % of the concentration indicated on the drug label. These tablets were also analyzed by high performance liquid chromatography (HPLC), obtaining a recovery of 103 % and LOD 4.0 x 10(-7) mol L-1. The velocity of analysis using flow methods compares advantageously to the classical chromatographic methods. For synthetic urine, linear responses were obtained in samples spiked in the region from 5.0 x 10(-6) mol L-1 to 30 x 10(-6) mol L-1 (R-2=0.991) and LOD 3.0 x 10(-7) mol L-1.

Automated summary

This study developed a fast, precise, and reliable voltammetric method for quantifying indapamide, an orally active diuretic sulfonamide used for hypertensive treatment. The method showed linear responses and low detection limits in both tablet and synthetic urine samples. The flow injection analysis technique used in this method proved to be advantageous compared to classical chromatographic methods in terms of analysis speed. The study also compared the results obtained by voltammetry with those obtained by high performance liquid chromatography (HPLC), and showed good correlation.