Transcriptomic and physiological properties reveal the tolerance mechanism to difenoconazole toxicity in wheat (Triticum aestivum L.)

Difenoconazole (DFZ) is a broad-spectrum fungicide widely applied in wheat production. However, excessive accumulation is linked to phytotoxicity. The effects of DFZ on plants and the response mechanisms to DFZ toxicity are poorly understood. Herein, the uptake, accumulation, and translocation of DFZ and induced changes in the morphology, physiology, and gene expression were investigated under hydroculture of roots treated with 50, 100, and 200 mg/L DFZ concentrations. Compared with the control, DEZ treatment upregulated the expression of genes encoding 4-coumarate-CoA ligase (4CL) and peroxidase (POD) involved in the lignin biosynthesis pathway and enhanced lignin biosynthesis. DFZ accumulated more in older leaves (cotyledons and lower true leaves), with 0.49-5.71 and 0.09-2.14 folds higher than levels in new upper leaves and roots, respectively. The excessive accumulation of DFZ in tissues was rapidly degraded, with a 15.7-69.3% reduction of DFZ content in roots and leaves from 3 DAT to 6 DAT. The genes expression and activity of glutathione S-transferase (GST) were increased. Furthermore, DFZ treatments upregulated genes encoding chalcone synthase (CHS), chalcone isomerase (CHI), and anthocyanidin synthase (ANS) involved in the flavonoid biosynthesis pathway and increased the amount of flavonoid and anthocyanins in leaves. This study provides new insights into the self-protective behaviors exhibited by wheat plants under DFZ stress. The mechanisms included hin-dering DFZ penetration from roots by enhancing lignin biosynthesis, accumulating more in old leaves, degrading by GST, and alleviating oxidative damage by increasing the content of flavonoids and anthocyanins in leaves.

Automated summary

This study investigated the uptake, accumulation, and translocation of difenoconazole (DFZ) in plants, as well as its effects on morphology, physiology, and gene expression. Results showed that DFZ predominantly accumulated in older leaves and wheat plants exhibited self-protective behaviors by enhancing lignin biosynthesis, degrading DFZ by glutathione S-transferase, and increasing the content of flavonoids and anthocyanins to alleviate oxidative damage.