4.4 Article

Fluorescence-Based High-Throughput Assays for Investigating Cytochrome P450 Enzyme-Mediated Drug-Drug Interactions

Journal

DRUG METABOLISM AND DISPOSITION
Volume 51, Issue 10, Pages 1254-1272

Publisher

AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/dmd.122.001068

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This review provides an overview of recent advances in the development of highly specific optical substrates for human CYP enzymes and their applications in screening CYP inhibitors and studying DDIs/HDIs. The review summarizes optical-based biochemical assays for efficient detection of CYP activities, which have greatly facilitated the discovery of CYP inhibitors and the investigation of CYP-mediated DDIs.
The cytochrome P450 enzymes (CYPs), a group of heme-containing enzymes, catalyze oxidative metabolism of a wide range of drugs and xenobiotics, as well as different endogenous molecules. Strong inhibi-tion of human CYPs is the most common cause of clinically associated pharmacokinetic drug-drug/herb-drug interactions (DDIs/HDIs), which may result in serious adverse drug reactions, even toxicity. Accurate and rapid assessing of the inhibition potentials on CYP activities for therapeutic agents is crucial for the prediction of clinically relevant DDIs/HDIs. Over the past few decades, significant efforts have been invested into developing optical substrates for the human CYPs, generating a variety of powerful tools for high-throughput assays to detect CYP activities in biologic specimens and for screening of CYP inhibitors. This minireview focuses on recent advances in optical substrates developments for human CYPs, as well as their applica-tions in screening CYP inhibitors and DDIs/HDIs studies. The exam-ples for rational design and optimization of highly specific optical substrates for the target CYP enzyme, as well as applications in inves-tigating CYP-mediated DDIs, are illustrated. Finally, the challenges and future perspectives in this field are proposed. Collectively, this review summarizes the reported optical-based biochemical assays for highly efficient CYP activities detection, which strongly facilitated the discovery of CYP inhibitors and the investigations on CYP-mediated DDIs.SIGNIFICANCE STATEMENT Optical substrates for cytochrome P450 enzymes (CYPs) have emerged as powerful tools for the construction of high-throughput assays for screening of CYP inhibitors. This mini-review covers the advances and challenges in the development of highly specific optical substrates for sensing human CYP isoenzymes, as well as their applications in con-structing fluorescence-based high-throughput assays for investigating CYP-mediated drug-drug interactions.

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