Intracellular Delivery of an Antibody-Like Molecule Capable of Inhibiting c-Myc

A modular nanotransporter (MNT) carrying the sequence of an antibody-like molecule, anti-c-Myc nanobody, was synthesized and characterized. It was demonstrated that the created MNT is able to interact with the target protein, c-Myc oncogene, with a dissociation constant of 46 +/- 14 nM, internalize into target cells, change Myc-dependent expression, and exert an antiproliferative effect.

Automated summary

A modular nanotransporter (MNT) carrying an antibody-like molecule, anti-c-Myc nanobody, was synthesized and characterized. It was found that the MNT could bind to the target protein, c-Myc oncogene, with a dissociation constant of 46 +/- 14 nM. Moreover, the MNT was able to internalize into target cells, alter Myc-dependent expression, and exhibit antiproliferative effects.