Journal
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
Volume 28, Issue 3, Pages 823-836Publisher
AMER SOC NEPHROLOGY
DOI: 10.1681/ASN.2015080898
Keywords
-
Categories
Funding
- Fondo Europeo de Desarrollo Regional funds
- Fondo de Investigacion en Salud Instituto de Salud Carlos III [PI13/00047, PI15/00298, CP14/00133, CP12/03262]
- Red Tematica de Investigacion Cooperativa en Salud
- Red de Investigacion [Renal RD12/0021]
- European Uremic Toxin Work Group
- Sociedad Espariola de Nefrologia
- Comunidad de Madrid [CIFRA S2010/BMD-2378]
- Fondecyt [1160465]
- FIS Miguel Servet
- Programa Intensificacion Actividad Investigadora (ISCIII/Agencia Lain-Entralgo/CM)
- FP7 program Improvement of tools and portability of MS-based clinical proteomics as applied to chronic kidney disease (Protoclin, PEOPLE-IAPP) [GA 251368]
- FP7 program Clinical and system-omics for the identification of the Molecular Determinants of established Chronic Kidney Disease (iMODE-CKD) [PEOPLE-ITN-GA-2013-608332]
- FP7 program Systems biology towards novel chronic kidney disease diagnosis and treatment [SysKID HEALTH-F2-2009-241544]
Ask authors/readers for more resources
An improved understanding of pathogenic pathways in AKI may identify novel therapeutic approaches. Previously, we conducted unbiased liquid chromatography-tandem mass spectrometry based protein expression profiling of the renal proteome in mice with acute folate nephropathy. Here, analysis of the dataset identified enrichment of pathways involving NF kappa B in the kidney cortex, and a targeted data mining approach identified components of the noncanonical NF kappa B pathway, including the upstream kinase mitogen-activated protein kinase kinase kinase 14 (MAP3K14), the NF kappa B DNA binding heterodimer ReIB/NFKB2, and proteins involved in NF kappa B2 p100 ubiquitination and proteasomal processing to p52, as upregulated. Immunohistochemistry localized MAP3K14 expression to tubular cells in acute folate nephropathy and human AKI. In vivo, kidney expression levels of NF kappa B2 p100 and p52 increased rapidly after folic acid injection, as did DNA binding of ReIB and NF kappa B2, detected in nuclei isolated from the kidneys. Compared with wild-type mice, MAP3K14 activity- deficient aly/aly (MAP3K14(aly/aly)) mice had less kidney dysfunction, inflammation, and apoptosis in acute folate nephropathy and less kidney dysfunction and a lower mortality rate in cisplatin-induced AKI. The exchange of bone marrow between wild-type and MAP3K14(aly/aly) mice did not affect the survival rate of either group after folic acid injection. In cultured tubular cells, MAP3K14 small interfering RNA targeting decreased inflammation and cell death. Additionally, cell culture and in vivo studies identified the chemokines MCP-1, RANTES, and CXCL10 as MAP3K14 targets in tubular cells. In conclusion, MAP3K14 promotes kidney injury through promotion of inflammation and cell death and is a promising novel therapeutic target.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available