Journal
DEVELOPMENT
Volume 150, Issue 12, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.201552
Keywords
Horizontal basal cell; Stem cell; Olfactory epithelium; Regeneration
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Horizontal basal cells (HBCs) play a crucial role in regenerating the damaged olfactory epithelium (OE) by differentiating into olfactory sensory neurons (OSNs) and other non-neuronal cell types. The Notch signaling pathway has a context-dependent effect on HBC activity following OE injury. This study shows that HBCs depend on an inversion of the Notch pathway to properly mediate OE regeneration.
Horizontal basal cells (HBCs) residing within severely damaged olfactory epithelium (OE) mediate OE regeneration by differentiating into odorant-detecting olfactory sensory neurons (OSNs) and other tissue supporting non-neuronal cell types. Depending on both tissue type and integrity, the Notch signaling pathway can either positively or negatively regulate resident stem cell activity. Although Notch1 specifies HBC dormancy in the uninjured OE, little is known about how HBCs are influenced by the Notch pathway following OE injury. Here, we show that HBCs depend on a functional inversion of the Notch pathway to appropriately mediate OE regeneration. At 24 h post-injury, HBCs enhance Notch1-mediated signaling. Moreover, at 3 days post-injury when the regenerating OE is composed of multiple cell layers, HBCs enrich both Notch1 and the Notch ligand, Dll1. Notably, HBC-specific Notch1 knockout increases HBC quiescence and impairs HBC differentiation into neuronal progenitors and OSNs. Interestingly, complete HBC knockout of Dll1 only decreases differentiation of HBC-derived OSNs. These data underscore the context-dependent nature of Notch signaling. Furthermore, they reveal that HBCs regulate their own neurogenic potential after OE injury.
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