Protein dynamics by the combination of high-speed AFM and computational modeling

High-speed atomic force microscopy (HS-AFM) allows direct observation of biological molecules in dynamic action. How-ever, HS-AFM has no atomic resolution. This article reviews recent progress of computational methods to infer high -resolution information, including the construction of 3D atom-istic structures, from experimentally acquired resolution-limited HS-AFM images.

Automated summary

High-speed atomic force microscopy (HS-AFM) allows direct observation of biological molecules in dynamic action, but it lacks atomic resolution. This article reviews recent progress in computational methods for inferring high-resolution information, such as the construction of 3D atomic structures, from experimentally acquired resolution-limited HS-AFM images.