☆ 4.5 Review

Integrated mass spectrometry strategy for functional protein complex discovery and structural characterization

CURRENT OPINION IN CHEMICAL BIOLOGY (2023)

Related references

Note: Only part of the references are listed.

Article Chemistry, Multidisciplinary

Discovery of lipid-mediated protein-protein interactions in living cells using metabolic labeling with photoactivatable clickable probes

Roman O. O. Fedoryshchak et al.

Summary: Protein-protein interactions (PPIs) play important roles in biology. However, there are limited methods to study interactions driven by specific post-translational modifications (PTMs). In this study, a panel of novel myristic acid analog probes were designed and synthesized to capture PTM-specific interactions. These probes successfully labeled NMT substrates in cells and formed covalent crosslinks with their interacting proteins, allowing the identification of known and novel interactors of myristoylated proteins.

CHEMICAL SCIENCE (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Genetically encoded chemical crosslinking of carbohydrate

Shanshan Li et al.

Summary: Protein-carbohydrate interactions are important in biological processes but challenging to study. This study engineered covalent linkages between proteins and carbohydrates, offering a solution to overcome the low affinity and weak strength of these interactions.

NATURE CHEMISTRY (2023)

Add to Collection

Review Chemistry, Physical

Protein Structure Prediction with Mass Spectrometry Data

Sarah E. Biehn et al.

Summary: Knowledge of protein structure is essential for understanding biological function and drug discovery. Structural mass spectrometry allows for quick elucidation of protein structure features, but computational techniques are needed to convert sparse mass spectrometry data into protein models.

ANNUAL REVIEW OF PHYSICAL CHEMISTRY (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Surface-induced Dissociation Mass Spectrometry as a Structural Biology Tool

Dalton T. Snyder et al.

Summary: Native mass spectrometry (nMS) is an important tool in structural biology, with great potential due to a range of ionization techniques and new hybrid ion mobility and mass spectrometry systems. This review focuses on the key features and advantages of surface collisions (surface-induced dissociation, SID) for probing connectivity in protein and nucleoprotein complexes, demonstrating the importance of SID and nMS in future structural elucidation of biological assemblies.

CHEMICAL REVIEWS (2022)

Add to Collection

Article Biochemistry & Molecular Biology

The AlphaFold Database of Protein Structures: A Biologist's Guide

Alessia David et al.

Summary: AlphaFold, a deep learning algorithm developed by DeepMind, recently released three-dimensional models of the entire human proteome to the scientific community. In this discussion, we explore the advantages, limitations, and unresolved challenges of the AlphaFold models from the perspective of a biologist.

JOURNAL OF MOLECULAR BIOLOGY (2022)

Add to Collection

Editorial Material Biochemical Research Methods

The impact of AlphaFold2 one year on

David T. Jones et al.

NATURE METHODS (2022)

Add to Collection

Article Biochemistry & Molecular Biology

AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models

Mihaly Varadi et al.

Summary: AlphaFold DB is an openly accessible database with high-accuracy protein-structure predictions, powered by DeepMind's AlphaFold v2.0. It provides programmatic access to a vast number of predicted structures and is expanding to cover more sequences.

NUCLEIC ACIDS RESEARCH (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Studying protein structure and function by native separation-mass spectrometry

Guusje van Schaick et al.

Summary: Alterations in protein structure can greatly impact biological function. Analytical techniques that maintain the conformation and function of proteins are crucial for studying changes in their higher order structure and establishing structure-function relationships. The coupling of native protein separations with mass spectrometry is a powerful approach for studying these aspects in a reliable, fast, and straightforward manner. Affinity-based separations are highlighted for obtaining both structural and functional information of proteins simultaneously.

NATURE REVIEWS CHEMISTRY (2022)

Add to Collection

Review Chemistry, Multidisciplinary

High-Resolution Native Mass Spectrometry

Sem Tamara et al.

Summary: Native mass spectrometry is a method for analyzing and characterizing macromolecules while preserving their native structural features. It has experienced significant growth in the past decade, especially in enhancing sensitivity and resolving power, and has a wide range of applications.

CHEMICAL REVIEWS (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Integration of Mass Spectrometry Data for Structural Biology

Hannah M. Britt et al.

Summary: Mass spectrometry is increasingly used in the study of protein structure and dynamics. Structural MS methods provide unique structural information, and computational strategies are essential for integrating diverse data. Combining structural MS with other techniques is often necessary to capture the full structural details of a system. The integration of structural MS data has been fruitful in solving the structures of complex protein assemblies.

CHEMICAL REVIEWS (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Expressed Protein Selenoester Ligation

Sameer S. Kulkarni et al.

Summary: This study presents a novel EPSL methodology for the semi-synthesis of modified proteins. The EPSL technology combines the rapid kinetics of ligation reactions between modified synthetic selenopeptides and protein aryl selenoesters, and in situ chemoselective deselenization to efficiently obtain target proteins at high concentrations.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Uncovering post-translational modification-associated protein-protein interactions

Shu Wang et al.

Summary: The translation article discusses how proteins in living systems expand their chemical space and functional repertoire through post-translational modifications (PTMs). These modifications often trigger unique protein-protein interactions (PPIs) and uncovering these interactions is essential for understanding the roles of PTMs in biology.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2022)

Add to Collection

Article Biochemistry & Molecular Biology

AlphaFold2 fails to predict protein fold switching

Devlina Chakravarty et al.

Summary: AlphaFold2 revolutionized protein structure prediction, but its predictions tend to be inaccurate for structurally heterogeneous proteins. Analysis of sequence variation showed that fold-switching regions have similar conservation rates to canonical single-fold proteins, while intrinsically disordered regions have lower prediction confidences.

PROTEIN SCIENCE (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Complementing machine learning-based structure predictions with native mass spectrometry

Timothy M. Allison et al.

Summary: The development of machine learning-based structure prediction algorithms has made it possible for the scientific community to generate accurate structural models of cellular protein machinery. However, predicting the structure of protein complexes may still require user input and experimental validation. Mass spectrometry can be used in conjunction with machine learning to uncover structural features of ligand interactions, homology models, and point mutations that may not be detectable by machine learning algorithms alone.

PROTEIN SCIENCE (2022)

Add to Collection

Article Biochemistry & Molecular Biology

The structural context of posttranslational modifications at a proteome-wide scale

Isabell Bludau et al.

Summary: The recent revolution in computational protein structure prediction has provided new insights into the study of the entire proteome. In this study, the researchers analyze posttranslational modifications (PTMs) of proteins to determine their structural context and investigate their potential regulatory sites. The analysis reveals global patterns of PTM occurrence and spatial coregulation of different types of PTMs.

PLOS BIOLOGY (2022)

Add to Collection

Review Chemistry, Multidisciplinary

Native Mass Spectrometry at the Convergence of Structural Biology and Compositional Proteomics

Kevin Jooss et al.

Summary: Biology is driven by molecular interactions, and the language of protein-level biology is still largely undiscovered or inferred. Mass spectrometry has evolved to preserve and characterize protein complexes, allowing for a better understanding of protein interactions and their roles in human diseases. The future of native mass spectrometry includes advancements in separations, top-down mass spectrometry, and integration with structural biology, enabling the study of larger and more complex bioassemblies.

ACCOUNTS OF CHEMICAL RESEARCH (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Symmetry of 4-Oxalocrotonate Tautomerase Trimers Influences Unfolding and Fragmentation in the Gas Phase

Sarah N. Sipe et al.

Summary: The recent discovery of asymmetric trimers in the tautomerase superfamily (TSF) adds structural diversity and reveals the higher stability of asymmetric trimers compared to symmetric ones. Utilizing native mass spectrometry and ultraviolet photodissociation allows for rapid and sensitive differentiation of symmetric and asymmetric trimers. The increased stability of asymmetric trimers may justify their evolution and concomitant diversification of the TSF, aiding in delineating the evolutionary history of the TSF.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

Add to Collection

Review Spectroscopy

Native top-down mass spectrometry for higher-order structural characterization of proteins and complexes

Ruijie Liu et al.

Summary: Progress in structural biology research has created a higher demand for powerful tools to characterize proteins and protein complexes. Native mass spectrometry (nMS), particularly native top-down mass spectrometry (nTDMS), has gained significant interest in the past decade. This review highlights recent advances in nTDMS for structural research of biological assemblies, focusing on the additional layers of information enabled by TDMS. It also discusses the challenges, potential, and perspectives of nTDMS methods for protein and protein assembly analysis.

MASS SPECTROMETRY REVIEWS (2022)

Add to Collection

Article Multidisciplinary Sciences

A physical wiring diagram for the human immune system

Jarrod Shilts et al.

Summary: In this study, we systematically mapped the direct protein interactions across a recombinant library that includes most of the surface proteins on human leukocytes, providing a high-confidence and quantitative view of the receptor wiring that connects human immune cells. By integrating our interactome with expression data, we identified trends in the dynamics of immune interactions and constructed a mathematical model that predicts cellular connectivity. We also developed an interactive multi-tissue single-cell atlas to infer immune interactions throughout the body and linked our receptor interactions to functional roles through protein stimulation and microscopy.

NATURE (2022)

Add to Collection

Article Biochemistry & Molecular Biology

A modification-centric assessment tool for the performance of chemoproteomic probes

Ji-Xiang He et al.

Summary: Chemoproteomics is an important technology for exploring fundamental biology and discovering new small-molecule-based therapies. pChem is a computational tool that can assess the performance of chemoproteomic probes by recognizing isotopically coded probe-derived modifications, calculating masses accurately, and localizing sites precisely. The tool exports reports that score the profiling efficiency, modification homogeneity, and proteome-wide residue selectivity of the tested probe.

NATURE CHEMICAL BIOLOGY (2022)

Add to Collection

Article Multidisciplinary Sciences

Protein shape sampled by ion mobility mass spectrometry consistently improves protein structure prediction

S. M. Bargeen Alam Turzo et al.

Summary: This study develops an algorithm to predict protein collision cross sections (CCS) and integrates experimental ion mobility data into Rosetta-based molecular modeling to predict protein structures from sequence.

NATURE COMMUNICATIONS (2022)

Add to Collection

Article Multidisciplinary Sciences

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Jasjot Singh et al.

Summary: Lysosomes are crucial organelles for degradation and metabolism regulation. Protein complexes play a decisive role in lysosomal function. This study investigates lysosomes and early endosomes using cross-linking mass spectrometry, revealing protein interactions, structures, and cargo of flotillin-mediated endocytosis.

NATURE COMMUNICATIONS (2022)

Add to Collection

Article Biochemistry & Molecular Biology

Motif-dependent immune co-receptor interactome profiling by photoaffinity chemical proteomics

Xiong Chen et al.

Summary: Identification of the tyrosine phosphorylation (pY)-dependent interactome of immune co-receptors is crucial for understanding signal pathways involved in immunotherapy. In this study, a photoaffinity-based chemical proteomic approach was used to dissect the motif-specific cytoplasmic interactomes of CD28, a critical immune co-receptor. The study identified specific interactions of phospholipase PLCG1 with the Y191 motif and the kinase PKCq with the previously undefined pY218 motif. This synthetic CD28(cyto)-based photoaffinity proteomic approach can be applied to study other immune co-receptors with multiple pY sites.

CELL CHEMICAL BIOLOGY (2022)

Add to Collection

Article Chemistry, Multidisciplinary

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Xinyuan He et al.

Summary: This study reports a genetic method to introduce an analog of phosphotyrosine (pTyr), p-carboxymethyl-l-phenylalanine (CMF), into mammalian cells. This nonhydrolyzable pTyr analog can eliminate the complexity caused by the interconversion between phosphorylated and non-phosphorylated isoforms of a protein, facilitating biological studies.

CHEMICAL COMMUNICATIONS (2022)

Add to Collection

Article Biochemistry & Molecular Biology

The structural context of posttranslational modifications at a proteome-wide scale

Isabell Bludau et al.

Summary: The recent advancement in computational protein structure prediction allows integration with large-scale experimental data, helping to determine the structural context of posttranslational modifications (PTMs) and identify potential regulatory sites. Global patterns of PTM occurrence across folded and intrinsically disordered regions have been uncovered, with a focus on distinguishing regulatory PTMs from those marking improperly folded proteins in the human proteome.

PLOS BIOLOGY (2022)

Add to Collection

Editorial Material Biochemistry & Molecular Biology

AI revolutions in biology The joys and perils of AlphaFold

Anastassis Perrakis et al.

Summary: AlphaFold is the most ground-breaking application of AI in science so far, promising to revolutionize structural biology, but caution is advised in its implementation.

EMBO REPORTS (2021)

Add to Collection

Article Multidisciplinary Sciences

Accurate protein structure prediction with hydroxyl radical protein footprinting data

Sarah E. Biehn et al.

Summary: HRPF combined with mass spectrometry can reveal protein structure, while a dynamics-driven algorithm can enhance the accuracy of structure prediction.

NATURE COMMUNICATIONS (2021)

Add to Collection

Article Multidisciplinary Sciences

Structural insights into Ubr1-mediated N-degron polyubiquitination

Man Pan et al.

Summary: This study reveals the mechanism of the N-degron pathway mediated by Ubr1, including key structural elements involved in the initiation and elongation steps of ubiquitination.

NATURE (2021)

Add to Collection

Article Chemistry, Analytical

Improving the performance of proteomic analysis via VAILase cleavage and 193-nm ultraviolet photodissociation

Binwen Sun et al.

Summary: The combination of VAILase and trypsin digestion with 193-nm UVPD and HCD improves the performance of bottom-up proteomics by enhancing proteome sequence coverage and identification reliability. UVPD generates a high diversity of fragment ion types, contributing to a significant improvement in the XCorr score for both trypsin- and VAILase-digested peptides.

ANALYTICA CHIMICA ACTA (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome

Edward L. Huttlin et al.

Summary: This study explores thousands of interactions between proteins, creating two essential cell-line specific interaction networks that help researchers understand the principles of proteome organization and unknown protein characteristics. Comparing different cell lines revealed extensive customization, linking core complexes with essential proteins and rewiring subnetworks to produce each cell's phenotype.

CELL (2021)

Add to Collection

Article Biochemistry & Molecular Biology

A tri-functional amino acid enables mapping of binding sites for posttranslational-modification-mediated protein-protein interactions

Jianwei Lin et al.

Summary: The study introduces a new method, ADdis-Cys-MS, that can successfully identify PTM readers and map their PTM recognition modules simultaneously. Using this method, several binding sites of reader-PTM interactions were identified, including a novel histone chaperone.

MOLECULAR CELL (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Intracellular Protein-Drug Interactions Probed by Direct Mass Spectrometry of Cell Lysates

Rivkah Rogawski et al.

Summary: Researchers have developed a method based on native mass spectrometry for analyzing drug uptake and target engagement in cellular contexts. This approach utilizes direct MS for label-free studies of protein-drug binding in human cells.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Add to Collection

Review Biochemistry & Molecular Biology

Recent progress in mass spectrometry-based strategies for elucidating protein-protein interactions

Teck Yew Low et al.

Summary: This review discusses the underlying principles, advantages, limitations and experimental considerations of emerging MS-based proteomics techniques for characterising protein-protein interactions. In addition, it briefly accounts for how these techniques are used to investigate the structural and functional properties of protein complexes, including their topology, stoichiometry, copy number and dynamics.

CELLULAR AND MOLECULAR LIFE SCIENCES (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Probing membrane protein-lipid interactions

Mark T. Agasid et al.

Summary: Structure determination of membrane proteins has revealed the significant roles of lipids in influencing protein architecture. It is widely accepted that lipids surrounding membrane proteins play crucial roles by modulating their conformational, structural, and functional properties. Recent advances in mass spectrometry have provided insights into lipid interactions, shedding light on the dynamic nature of lipid-mediated interactions and their effects on conformation, interactions, and signaling.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2021)

Add to Collection

Article Multidisciplinary Sciences

Highly accurate protein structure prediction with AlphaFold

John Jumper et al.

Summary: Proteins are essential for life, and accurate prediction of their structures is a crucial research problem. Current experimental methods are time-consuming, highlighting the need for accurate computational approaches to address the gap in structural coverage. Despite recent progress, existing methods fall short of atomic accuracy in protein structure prediction.

NATURE (2021)

Add to Collection

Article Chemistry, Multidisciplinary

A proteome-wide atlas of lysine-reactive chemistry

Mikail E. Abbasov et al.

Summary: Recent advances in chemical proteomics have identified and characterized a wide range of uncharted aminophilic chemotypes that greatly expand the ligandable lysines in human proteins. Aminophilic electrophiles showed varying proteomic reactivities, from selective interactions with specific lysines to broad engagement with covalent small-molecule-lysine interactions. The study demonstrates the potential of covalent chemistry in targeting functional lysines in the human proteome, perturbing diverse biochemical functions.

NATURE CHEMISTRY (2021)

Add to Collection

Article Multidisciplinary Sciences

Accurate prediction of protein structures and interactions using a three-track neural network

Minkyung Baek et al.

Summary: Through the three-track network, we achieved accuracies approaching those of DeepMind in CASP14, enabling rapid solution of challenging x-ray crystallography and cryo-electron microscopy structure modeling problems, and providing insights into the functions of proteins with currently unknown structure.

SCIENCE (2021)

Add to Collection

Review Chemistry, Analytical

Mass Spectrometry-Based Protein Complex Profiling in Time and Space

Jiangnan Zheng et al.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Review Chemistry, Multidisciplinary

Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules

Jennifer S. Brodbelt et al.

CHEMICAL REVIEWS (2020)

Add to Collection

Article Biochemical Research Methods

Direct characterization of overproduced proteins by native mass spectrometry

Shay Vimer et al.

NATURE PROTOCOLS (2020)

Add to Collection

Article Biochemical Research Methods

Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry

Zachary L. VanAernum et al.

NATURE PROTOCOLS (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Cysteine/Penicillamine Ligation Independent of Terminal Steric Demands for Chemical Protein Synthesis

Yi Tan et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Add to Collection

Article Chemistry, Multidisciplinary

In situ chromatin interactomics using a chemical bait and trap approach

Antony J. Burton et al.

NATURE CHEMISTRY (2020)

Add to Collection

Article Biochemical Research Methods

Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins

Joseph Gault et al.

NATURE METHODS (2020)

Add to Collection

Article Cell Biology

Site-Specific Incorporation of Two ncAAs for Two-Color Bioorthogonal Labeling and Crosslinking of Proteins on Live Mammalian Cells

Birthe Meineke et al.

CELL REPORTS (2020)

Add to Collection

Review Chemistry, Multidisciplinary

Chemical Protein Synthesis: Advances, Challenges, and Outlooks

Yi Tan et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry

Mowei Zhou et al.

CHEMICAL SCIENCE (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase

Xiaojun Wu et al.

CELL CHEMICAL BIOLOGY (2020)

Add to Collection

Article Biochemical Research Methods

Initial Protein Unfolding Events in Ubiquitin, Cytochrome c and Myoglobin Are Revealed with the Use of 213 nm UVPD Coupled to IM-MS

Alina Theisen et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2019)

Add to Collection

Article Biochemical Research Methods

Native Top-Down Mass Spectrometry and Ion Mobility Spectrometry of the Interaction of Tau Protein with a Molecular Tweezer Assembly Modulator

Michael Nshanian et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2019)

Add to Collection

Review Biochemistry & Molecular Biology

Weighing-up protein dynamics: the combination of native mass spectrometry and molecular dynamics simulations

Erik G. Marklund et al.

CURRENT OPINION IN STRUCTURAL BIOLOGY (2019)

Add to Collection

Review Biochemistry & Molecular Biology

Membrane Protein-Lipid Interactions Probed Using Mass Spectrometry

Jani Reddy Bolla et al.

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88 (2019)

Add to Collection

Article Multidisciplinary Sciences

A high-speed search engine pLink 2 with systematic evaluation for proteome-scale identification of cross-linked peptides

Zhen-Lin Chen et al.

NATURE COMMUNICATIONS (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Predicting Protein Complex Structure from Surface-Induced Dissociation Mass Spectrometry Data

Justin T. Seffernick et al.

ACS CENTRAL SCIENCE (2019)

Add to Collection

Article Biochemistry & Molecular Biology

Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites

Alicia Lundby et al.

CELL (2019)

Add to Collection

Article Biochemistry & Molecular Biology

Genetic recoding to dissect the roles of site-specific protein O-GIcNAcylation

Andrii Gorelik et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2019)

Add to Collection

Review Chemistry, Analytical

Surface-Induced Dissociation: An Effective Method for Characterization of Protein Quaternary Structure

Alyssa Q. Stiving et al.

ANALYTICAL CHEMISTRY (2019)

Add to Collection

Article Multidisciplinary Sciences

Native mass spectrometry reveals the conformational diversity of the UVR8 photoreceptor

Ines S. Camacho et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Investigation of GTP-dependent dimerization of G12X K-Ras variants using ultraviolet photodissociation mass spectrometry

M. Rachel Mehaffey et al.

CHEMICAL SCIENCE (2019)

Add to Collection

Article Biochemistry & Molecular Biology

Top-down characterization of endogenous protein complexes with native proteomics

Owen S. Skinner et al.

NATURE CHEMICAL BIOLOGY (2018)

Add to Collection

Article Chemistry, Multidisciplinary

Elucidating the various multi-phosphorylation statuses of protein functional regions by 193-nm ultraviolet photodissociation

Zheyi Liu et al.

CHINESE CHEMICAL LETTERS (2018)

Add to Collection

Review Biochemistry & Molecular Biology

Collision induced unfolding of isolated proteins in the gas phase: past, present, and future

Sugyan M. Dixit et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2018)

Add to Collection

Article Multidisciplinary Sciences

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity

Kuan-Chuan Pao et al.

NATURE (2018)

Add to Collection

Article Chemistry, Multidisciplinary

An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes

Huilin Li et al.

NATURE CHEMISTRY (2018)

Add to Collection

Article Multidisciplinary Sciences

Dissecting ribosomal particles throughout the kingdoms of life using advanced hybrid mass spectrometry methods

Michiel van de Waterbeemd et al.

NATURE COMMUNICATIONS (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Akt Kinase Activation Mechanisms Revealed Using Protein Semisynthesis

Nam Chu et al.

CELL (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Mutations in Disordered Regions Can Cause Disease by Creating Dileucine Motifs

Katrina Meyer et al.

CELL (2018)

Add to Collection

Review Chemistry, Multidisciplinary

Native chemical ligation in protein synthesis and semi-synthesis

Anne C. Conibear et al.

CHEMICAL SOCIETY REVIEWS (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Genetically Encoded Protein Phosphorylation in Mammalian Cells

Vaclav Beranek et al.

CELL CHEMICAL BIOLOGY (2018)

Add to Collection

Article Biochemical Research Methods

Native Mass Spectrometry: What is in the Name?

Aneika C. Leney et al.

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2017)

Add to Collection

Article Chemistry, Multidisciplinary

Genetically Encoded Photoaffinity Histone Marks

Xiao Xie et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Add to Collection

Article Multidisciplinary Sciences

The role of interfacial lipids in stabilizing membrane protein oligomers

Kallol Gupta et al.

NATURE (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria

Xiaozhou Luo et al.

NATURE CHEMICAL BIOLOGY (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Site-specific incorporation of phosphotyrosine using an expanded genetic code

Christian Hoppmann et al.

NATURE CHEMICAL BIOLOGY (2017)

Add to Collection

Article Biochemical Research Methods

Biosynthesis and genetic encoding of phosphothreonine through parallel setection and deep sequencing

Michael Shaofei Zhang et al.

NATURE METHODS (2017)

Add to Collection

News Item Multidisciplinary Sciences

Unique free electron laser laboratory opens in China

Dennis Normile

SCIENCE (2017)

Add to Collection

Review Chemistry, Analytical

Ion Activation Methods for Peptides and Proteins

Jennifer S. Brodbelt

ANALYTICAL CHEMISTRY (2016)

Add to Collection

Article Biochemistry & Molecular Biology

Photo-lysine captures proteins that bind lysine post-translational modifications

Tangpo Yang et al.

NATURE CHEMICAL BIOLOGY (2016)

Add to Collection

Article Chemistry, Multidisciplinary

Impact of G12 Mutations on the Structure of K-Ras Probed by Ultraviolet Photodissociation Mass Spectrometry

Michael B. Cammarata et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)

Add to Collection

Review Multidisciplinary Sciences

Mass-spectrometric exploration of proteome structure and function

Ruedi Aebersold et al.

NATURE (2016)

Add to Collection

Article Chemistry, Analytical

Surface-Induced Dissociation Mass Spectra as a Tool for Distinguishing Different Structural Forms of Gas-Phase Multimeric Protein Complexes

Royston S. Quintyn et al.

ANALYTICAL CHEMISTRY (2015)

Add to Collection

Article Chemistry, Physical

Amyloid β-Protein Assembly: The Effect of Molecular Tweezers CLR01 and CLR03

Xueyun Zheng et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2015)

Add to Collection

Article Biochemistry & Molecular Biology

Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog

Daniel T. Rogerson et al.

NATURE CHEMICAL BIOLOGY (2015)

Add to Collection

Article Chemistry, Multidisciplinary

Structural characterization of holo- and apo-myoglobin in the gas phase by ultraviolet photodissociation mass spectrometry

Michael B. Cammarata et al.

CHEMICAL SCIENCE (2015)

Add to Collection

Article Chemistry, Multidisciplinary

Dynamics of Intact Immunoglobulin G Explored by Drift-Tube Ion-Mobility Mass Spectrometry and Molecular Modeling

Kamila J. Pacholarz et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)

Add to Collection

Article Chemistry, Multidisciplinary

Characterization of Native Protein Complexes Using Ultraviolet Photodissociation Mass Spectrometry

John P. O'Brien et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2014)

Add to Collection

Article Multidisciplinary Sciences

Membrane proteins bind lipids selectively to modulate their structure and function

Arthur Laganowsky et al.

NATURE (2014)

Add to Collection

Article Biochemical Research Methods

A mass spectrometry-based hybrid method for structural modeling of protein complexes

Argyris Politis et al.

NATURE METHODS (2014)

Add to Collection

Review Chemistry, Multidisciplinary

Protein Analysis by Shotgun/Bottom-up Proteomics

Yaoyang Zhang et al.

CHEMICAL REVIEWS (2013)

Add to Collection

Article Chemistry, Multidisciplinary

Complete Protein Characterization Using Top-Down Mass Spectrometry and Ultraviolet Photodissociation

Jared B. Shaw et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Add to Collection

Article Biochemistry & Molecular Biology

A Map of General and Specialized Chromatin Readers in Mouse Tissues Generated by Label-free Interaction Proteomics

H. Christian Eberl et al.

MOLECULAR CELL (2013)

Add to Collection

Article Multidisciplinary Sciences

Temporal regulation of EGF signalling networks by the scaffold protein Shc1

Yong Zheng et al.

NATURE (2013)

Add to Collection

Article Biochemical Research Methods

Chemical synthesis of proteins using peptide hydrazides as thioester surrogates

Ji-Shen Zheng et al.

NATURE PROTOCOLS (2013)

Add to Collection

Review Chemistry, Multidisciplinary

Mass spectrometry-based proteomics: existing capabilities and future directions

Thomas E. Angel et al.

CHEMICAL SOCIETY REVIEWS (2012)

Add to Collection

Review Chemistry, Multidisciplinary

Ion mobility mass spectrometry of proteins and protein assemblies

Charlotte Uetrecht et al.

CHEMICAL SOCIETY REVIEWS (2010)

Add to Collection

Review Biochemistry & Molecular Biology

Target profiling of small molecules by chemical proteomics

Uwe Rix et al.

NATURE CHEMICAL BIOLOGY (2009)

Add to Collection

Article Multidisciplinary Sciences

Stepwise evolution of protein native structure with electrospray into the gas phase, 10-12 to 102 S

Kathrin Breuker et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Add to Collection

Review Multidisciplinary Sciences

Dynamic personalities of proteins

Katherine Henzler-Wildman et al.

NATURE (2007)

Add to Collection

Article Chemistry, Multidisciplinary

Top-down ESI-ECD-FT-ICR mass spectrometry localizes noncovalent protein-ligand binding sites

Yongming Xie et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2006)

Add to Collection

Article Biochemistry & Molecular Biology

Phosphotyrosine interactome of the ErbB-receptor kinase family

Waltraud X. Schulze et al.

MOLECULAR SYSTEMS BIOLOGY (2005)

Add to Collection

© Peeref 2019-2024. All rights reserved.