Journal
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
Volume -, Issue -, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10408347.2023.2180319
Keywords
Biochemical analysis; bioreactor; biosensors; gene modular; synthetic biology
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In recent years, synthetic biologists have developed biological elements and bioreactors composed of nucleotides using engineering methods. This paper reviews the biosensor components based on synthetic bioreactors and reporters, and discusses their applications in detecting heavy metal ions, nucleic acid, antibiotics, and other substances using cell and cell-free systems. It also examines the current bottlenecks faced by biosensors and potential directions for optimization.
In the past few years, synthetic biologists have established some biological elements and bioreactors composed of nucleotides under the guidance of engineering methods. Following the concept of engineering, the common bioreactor components in recent years are introduced and compared. At present, biosensors based on synthetic biology have been applied to water pollution monitoring, disease diagnosis, epidemiological monitoring, biochemical analysis and other detection fields. In this paper, the biosensor components based on synthetic bioreactors and reporters are reviewed. In addition, the applications of biosensors based on cell system and cell-free system in the detection of heavy metal ions, nucleic acid, antibiotics and other substances are presented. Finally, the bottlenecks faced by biosensors and the direction of optimization are also discussed.
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