FRET-Calc: A free software and web server for F?rster Resonance Energy Transfer Calculation

Forster Resonance Energy Transfer Calculator (FRET-Calc) is a program and web server that analyzes molar extinction coefficient of the acceptor, emission spectrum of the donor, and the refractive index spectrum of the donor/acceptor blend. Its main function is to obtain important parameters of the FRET process from experimental data, such as: (i) effective refractive index, (ii) overlap integral, (iii) Forster radius, (iii) FRET efficiency and (iv) FRET rate. FRET-Calc is license free software that can be run via dedicated web server (nanocalc.org) or downloading the program executables (for Unix, Windows, and macOS) from the FRET-Calc repository on GitHub. The program features a user-friendly interface, making it suitable for materials research and teaching purposes. In addition, the program is optimized to run on normal computers and is lightweight. An example will be given with the step by step of its use and results obtained.(c) 2023 Elsevier B.V. All rights reserved.

Automated summary

FRET-Calc is a program and web server that analyzes various parameters of the FRET process, such as effective refractive index, overlap integral, Förster radius, FRET efficiency, and FRET rate. It can be accessed through a web server or downloaded as software for different operating systems. The program is user-friendly and lightweight, making it suitable for both research and teaching purposes. A step-by-step example and results are provided.