Designing liquefaction and saccharification processes of highly concentrated starch slurry: Challenges and recent advances

Starch-based sugars are an important group of starch derivatives used in food, medicine, chemistry, and other fields. The production of starch sugars involves starch liquefaction and saccharification processes. The production cost of starch sugars can be reduced by increasing the initial concentration of starch slurry. However, the usage of the highly concentrated starch slurry is characterized by challenges such as low reaction efficiency and poor product performance during the liquefaction and saccharification processes. In this study, we endeavored to provide a reference guide for improving high-concentration starch sugar production. Thus, we reviewed the effects of substrate concentration on the starch sugar production process and summarized several potential strategies. These regulation strategies, such as physical field pretreatment, complex enzyme-assisted, and temperature control, can significantly increase the starch concentration and mitigate the challenges of using highly concentrated starch slurry. We believe that highly concentrated starch sugar production will achieve a qualitative leap in the future. This review provides theoretical guidance and highlights the importance of high concentration in starch-based sugar production. Further studies are needed to explore the fine structure and enzyme attack mode during the liquefaction and saccharification processes to regulate the production of more targeted products.

Automated summary

Starch-based sugars play a crucial role in various industries including food, medicine, and chemistry. Increasing the initial concentration of starch slurry can reduce the production cost, but it presents challenges such as low reaction efficiency and poor product performance. This study reviews the effects of substrate concentration on starch sugar production and proposes several regulation strategies like pretreatment, enzyme-assisted methods, and temperature control to overcome these challenges. The future of highly concentrated starch sugar production looks promising, and further research is needed to understand the structure and enzyme attack mode for targeted product production.