Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 223, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2023.113152
Keywords
Strontium oxide nanoceramics; Biomaterials; Tissue engineering; Antibacterial; Osteogenesis
Ask authors/readers for more resources
Fabricating bioartificial bone graft ceramics with properties similar to native stem-cell niches is challenging. Infections caused by microbial invasion are also a concern. In this study, biomimetic porous scaffolds were developed, which significantly improved biocompatibility, osteo-regeneration, mechanical strength, and antibacterial properties. These scaffolds showed superior performance compared to conventional scaffolds, making them promising for bone tissue regeneration.
Fabricating bioartificial bone graft ceramics retaining structural, mechanical, and bone induction properties akin to those of native stem-cell niches is a major challenge in the field of bone tissue engineering and regenerative medicine. Moreover, the developed materials are susceptible to microbial invasion leading to biomaterialcentered infections which might limit their clinical translation. Here, we successfully developed biomimetic porous scaffolds of polyurethane-reinforcedL-cysteine-anchored polyaniline capped strontium oxide nanoparticles to improve the scaffold's biocompatibility, osteo-regeneration, mechanical, and antibacterial properties. The engineered nanocomposite substrate PU/L-Cyst-SrO2 @PANI (0.4 wt%) significantly promotes bone repair and regeneration by modulating osteolysis and osteogenesis. ALP activity, collagen-I, ARS staining, as well as biomineralization of MC3T3-E1 cells, were used to assess the biocompatibility and cytocompatibility of the developed scaffolds in vitro, confirming that the scaffold provided a favorable microenvironment with a prominent effect on cell growth, proliferation, and differentiation. Furthermore, osteogenic protein markers were studied using qRT-PCR with expression levels of runt-related transcription factor 2 (RUNX2), secreted phosphoprotein 1 (Spp-I), and collagen type I (Col-I). The overall results suggest that PU/L-Cyst-SrO2 @PANI (0.4 wt %) scaffolds showed superior interfacial biocompatibility, antibacterial properties, load-bearing ability, and osteoinductivity as compared to pristine PU. Thus, prepared bioactive nanocomposite scaffolds perform as a promising biomaterial substrate for bone tissue regeneration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available