4.4 Article

Senescent CD4+CD28null cells are increased in chronic hyperuricemia, show aberrant effector phenotypes, and are reversed after allopurinol therapy: a proof-of-concept pilot study

Journal

CLINICAL RHEUMATOLOGY
Volume 42, Issue 8, Pages 2181-2186

Publisher

SPRINGER LONDON LTD
DOI: 10.1007/s10067-023-06595-8

Keywords

Allopurinol; CD28; Hyperuricemia; Inflammation; T cells

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This study characterized CD4(+)CD28(null) cells in patients with chronic hyperuricemia and investigated the effect of allopurinol on CD28 expression and the balance of T helper phenotypes. The results showed that hyperuricemic patients had an expanded CD4(+)CD28(null) cell subset with an increase in T-bet(+) cells and a decrease in ROR?t(+) cells. After allopurinol treatment, CD4(+)CD28(null) cells decreased, T-bet(+) cells decreased, GATA-3(+) cells increased, and ROR?t(+) cells increased. These findings suggest that allopurinol partially restores CD28 expression and rebalances T helper phenotypes in chronic hyperuricemia patients.
To characterize CD4(+)CD28(null) cells in chronic hyperuricemia and investigate whether allopurinol could restore CD28 expression and the balance of T helper phenotypes. Asymptomatic individuals with chronic hyperuricemia and ultrasonographic findings evocative of urate deposition in the joints. Age- and gender-matched normouricemic individuals were also studied. Oral allopurinol at 150 mg/day for 4 weeks, followed by 300 mg/day through week 12. Color-flow cytometry on peripheral blood mononuclear cells (PBMC) with antibodies against CD4, CD28, T-bet (Th1), GATA-3 (Th2), and ROR?t (Th17). Six patients (five men, median age of 53 years) and seven controls were studied. At baseline, hyperuricemic patients had more CD4(+)CD28(null)/CD4(+) cells than normouricemic subjects (36.8% vs. 6.1%; p = 0.001), with a predominance of T-bet(+) cells (98.5% vs. 6.6%; p = 0.001) and few ROR?t(+) cells (0.7% vs. 89.4%; p = 0.014). In hyperuricemic patients, the number of CD4(+) cells/10,000 PBMC was similar before and after allopurinol (3378 vs. 3954; p = 0.843). Conversely, CD4(+)CD28(null) cells decreased from 36.8% (23.0-43.7) to 15.8% (4.7-28.1; p = 0.031). CD4(+)CD28(null)T-bet(+) cells decreased from 98.5% (95.0-99.4) to 88.3% (75.2-98.9; p = 0.062), CD4(+)CD28(null)GATA-3(+) cells increased from 0% (0-4.0) to 2.8% (0.1-15.6; p = 0.156), and CD4(+)CD28(null)ROR?t(+) cells increased from 0.7% (0.4-7.0) to 4.5% (1.3-28.1; p = 0.031). The CD4(+)CD28(null) cell subset is abnormally expanded in chronic hyperuricemia, despite the absence of overt urate-related disease. Allopurinol may partially restore CD28 expression on CD4(+) cells while enhancing the homeostatic balance of T helper phenotypes. ClinicalTrials.gov, number NCT04012294.

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