Journal
CHIRALITY
Volume 35, Issue 7, Pages 427-434Publisher
WILEY
DOI: 10.1002/chir.23552
Keywords
circular dichroism (CD) spectroscopy; ganirelix acetate; humidity; secondary structure; temperature
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Ganirelix, a drug used in IVF, inhibits ovulation by blocking the production of gonadotropins. Peptides are macromolecules that can maintain their predetermined shape and carry out their intended functions. Changes in temperature and humidity can alter the secondary structure of therapeutic peptides. Circular dichroism spectroscopy was used to evaluate the effect of environmental factors on Ganirelix's secondary structure. The results showed that the stressed drug products had similar secondary structures to the reference drug.
Ganirelix, a drug used in in vitro fertilization (IVF), prevents ovulation in women who are not ready to have children by inhibiting a gene that produces gonadotropin. Peptides are macromolecules that are able to preserve a predetermined shape while carrying out the structural and regulatory roles for which they were originally intended. Peptide structures can be altered in the production and storage processes. Therapeutic peptides' biological activity can be drastically altered by even small modifications in their primary and secondary structures. The molecules' secondary structures can be monitored by subjecting them to different processing or storage conditions. In our investigation, we used circular dichroism (CD) spectroscopy with two different software programs for secondary structure evaluation to look at how environmental factors like temperature and humidity affected the secondary structure of Ganirelix in an injectable formulation. The CD results revealed that the alpha-helical (regular and distorted), beta-sheet, beta-strands (regular and distorted), beta-turn, and random coil structures of temperature and humidity stressed generic drug products are comparable to reference-listed drug.
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