Exploring Glycosylated Soy Isoflavones Affinities toward G-tetrads as Studied by Survival Yield Method

Taking soy-based food supplements for menopausal symptoms by women may reduce the risk of cancer. Therefore, the interaction between nucleic acids (or their constituents) and ingredients of the supplements, e. g., isoflavone glucosides, on the molecular level, has been of interest with respect to cancer therapy. In this work, the interaction between isoflavone glucosides and G-tetrads, namely [4G+Na](+) ions (G stands for guanosine or deoxyguanosine), were analyzed by using electrospray ionization-collision induced dissociation-mass spectrometry (ESI-CID-MS) and survival yields method. The strength of isoflavone glucosides-[4G+Na](+) interaction in the gas phase was determined from E-com50 - the energy required to fragment 50 % of selected precursor ions. Glycitin-[4G+Na](+) interaction was found to be the strongest, and the interaction between isoflavone glucosides and guanosine tetrad was established to be stronger than that between isoflavone glucosides and deoxyguanosine tetrad.

Automated summary

The interaction between soy-based food supplements for menopausal symptoms and nucleic acids has been studied to understand its potential impact on cancer therapy. By using ESI-CID-MS and survival yields method, the strength of the interaction between isoflavone glucosides and G-tetrads was analyzed. The results showed that the interaction between glycitin and [4G+Na](+) ions was the strongest, indicating its potential role in cancer prevention.