A novel atropine electrochemical sensor based on silver nano particle-coated Spirulina platensis multicellular blue-green microalga

In this work, Atropine as the anticholinergic drug was measured using the environmentally friendly sensor. In this regard, Self-cultivated Spirulina platensis with electroless silver was employed as a powder amplifier in carbon paste electrode modification. Also, 1-Hexyl-3 methylimidazolium Hexafluorophosphate (HMIM PF6) ion liquid as a conductor binder was used in the suggested electrode construction. Atropine determination was investigated by voltammetry methods. According to voltammograms, the electrochemical behavior of atropine depends on pH, and pH 10.0 was used as the optimal condition. Moreover, the diffusion control process for the electro-oxidation of atropine was verified by the scan rate study, so the diffusion coefficient (D similar to 3.0136x10(-4) cm(2)/sec) value was computed from the chronoamperometry study. Furthermore, responses of the fabricated sensor were linear in the concentration range from 0.01 to 800 mu M, and the lowest detection limit of the Atropine determination was obtained at 5 nM. Moreover, the stability, reproducibility, and selectivity factors of the suggested sensor were confirmed by the results. Finally, the recovery percentages for atropine sulfate ampoule (94.48-101.58), and water (98.01-101.3) approve of the applicability of the proposed sensor to Atropine determination in real samples.

Automated summary

In this study, an environmentally friendly sensor was used to measure the anticholinergic drug Atropine. Self-cultivated Spirulina platensis with electroless silver and 1-Hexyl-3 methylimidazolium Hexafluorophosphate (HMIM PF6) ion liquid were employed as materials for electrode modification. Voltammetry methods were used to determine Atropine, and pH 10.0 was found to be the optimal condition. The fabricated sensor showed linear responses in the concentration range from 0.01 to 800 μM, with a lowest detection limit of 5 nM. The stability, reproducibility, and selectivity of the sensor were confirmed, and it was successfully applied to the determination of Atropine in real samples.