Genome-wide association studies and transcriptome sequencing analysis reveal novel genes associated with Al tolerance in wheat

Aluminum (Al) toxicity is a major threat to the productivity and quality of wheat on acid soil. Identifying novel Al tolerance genes is crucial for breeders to pyramid different tolerance mechanisms thus leading to greater Al tolerance. We aim to identify novel quantitative trait loci (QTL) and key candidate genes associated with Al tolerance in wheat. Herein, we investigated the genotypic variation in Al tolerance among 334 wheat varieties using an acid soil assay. Genome-wide association study (GWAS) and transcriptome were carried out to identify key genes for Al tolerance. GWAS identified several QTL associated with acid soil tolerance including one major QTL on chromosome 1A, in addition to the QTL on 4D where TaALMT1 is located. The four significant markers around the newly identified QTL explained 27.2% of the phenotypic variation. With the existence of reported markers for TaALMT1, more than 97% of the genotypes showed tolerance to Al. For those genotypes with the existence of the novel QTL on 1A but without TaALMT1, more than 90% of genotypes showed medium or high tolerance to Al, confirming the existence of the Al tolerance gene(s) on chromosome 1A. By combining GWAS and RNA-seq analysis, we identified 11 candidate genes associated with Al tolerance. The results provide new insights into the genetic basis of Al tolerance in wheat. The identified genes can be used for the breeding of Al tolerant accessions.

Automated summary

Aluminum toxicity poses a significant threat to wheat productivity and quality on acid soil. This study aims to identify novel genes and genetic loci associated with aluminum tolerance in wheat through genome-wide association study (GWAS) and transcriptome analysis. The results reveal several QTL associated with acid soil tolerance, including a major QTL on chromosome 1A and the known TaALMT1 gene on chromosome 4D. By combining GWAS and RNA-seq analysis, 11 candidate genes related to aluminum tolerance were identified. These findings provide valuable insights into the genetic basis of aluminum tolerance in wheat and can aid in the breeding of tolerant varieties.