Activity Sensing of Coagulation and Fibrinolytic Proteases

The blood coagulation cascade is a complex physiological process involving the action of multiple coupled enzymes, cofactors, and substrates, ultimately leading to clot formation. Serine proteases have a crucial role, and aberrations in their activity can lead to life-threatening bleeding disorders and thrombosis. This review summarizes the essential proteases involved in blood coagulation and fibrinolysis, the endogenous peptide sequences they recognize and hydrolyze, and synthetic peptide probes based on these sequences to measure their activity. The information in this review can contribute to developing novel anticoagulant therapies and specific substrates for point-of-care diagnosis of coagulation pathologies.

Automated summary

The blood coagulation cascade is a complex physiological process that involves multiple enzymes, cofactors, and substrates, leading to clot formation. Serine proteases play a crucial role, and abnormalities in their activity can result in life-threatening bleeding disorders and thrombosis. This review summarizes the important proteases involved in blood coagulation and fibrinolysis, their recognition and hydrolysis of endogenous peptide sequences, and synthetic peptide probes for measuring their activity. The information in this review can contribute to the development of novel anticoagulant therapies and specific substrates for point-of-care diagnosis of coagulation pathologies.