Journal
CELL PROLIFERATION
Volume 56, Issue 5, Pages -Publisher
WILEY
DOI: 10.1111/cpr.13481
Keywords
-
Categories
Ask authors/readers for more resources
Regeneration is a vital process for repairing damaged tissues or organs, from primitive organisms to higher mammals. Planarian, with its extensive reservoir of adult stem cells, provides an ideal model to study regeneration. The depletion of m(6)A methyltransferase regulatory subunit wtap abolishes planarian regeneration, potentially by regulating genes related to cell-cell communication and cell cycle.
Regeneration is the regrowth of damaged tissues or organs, a vital process in response to damages from primitive organisms to higher mammals. Planarian possesses active whole-body regenerative capability owing to its vast reservoir of adult stem cells, neoblasts, providing an ideal model to delineate the underlying mechanisms for regeneration. RNA N-6-methyladenosine (m(6)A) modification participates in many biological processes, including stem cell self-renewal and differentiation, in particular the regeneration of haematopoietic stem cells and axons. However, how m(6)A controls regeneration at the whole-organism level remains largely unknown. Here, we demonstrate that the depletion of m(6)A methyltransferase regulatory subunit wtap abolishes planarian regeneration, potentially through regulating genes related to cell-cell communication and cell cycle. Single-cell RNA-seq (scRNA-seq) analysis unveils that the wtap knockdown induces a unique type of neural progenitor-like cells (NP-like cells), characterized by specific expression of the cell-cell communication ligand grn. Intriguingly, the depletion of m(6)A-modified transcripts grn, cdk9 or cdk7 partially rescues the defective regeneration of planarian caused by wtap knockdown. Overall, our study reveals an indispensable role of m(6)A modification in regulating whole-organism regeneration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available