Ag Nanoparticles Stabilized on Magnetic Carboxymethyl Lignin: Synthesis, Characterization and its Performance in the N-Acylation Reactions and Investigation of its Antioxidant and Anti-Human Colorectal Cancer Application

This report expresses a sustainable methodology for the in situ synthesis of Ag nanoparticles (NPs) adorned carboxymethyl lignin (CML) functionalized Fe3O4 nanocomposite (Fe3O4@CML/Ag) and its subsequent chemical and biological applications. Structural and physicochemical features of such novel material was evaluated through a number of analytical techniques like FT-IR, FE-SEM, TEM, EDS, XRD, ICP and VSM. The nanomaterial was a competent catalyst towards the N-acylation of diverse amines using acetic anhydride at solvent-free conditions. The material was isolated by magnet and reused for 8 consecutive times. Furthermore, the material was employed in the biological evaluation of anti-oxidant properties by DPPH method as well as the anticancer studies of against HT-29 and Caco-2 colon cell lines following MTT method. The DPPH assay afforded a significant IC50 value that suggested the material to have good anticancer properties. Interestingly, % cell viability of the malignant cell lines was found to decrease dose-dependently over Fe3O4@NaLS/Ag nanocomposite. MTT assay afforded the corresponding IC50 values to be 395.4 mu g/ml and 287.3 mu g/ml respectively against the two cell lines. The absorbance rate was evaluated at 545 nm, which represented toxicity on normal cell line (CHO) even up to 2000 mu g/mL. [GRAPHICS] .

Automated summary

This report introduces a sustainable method for synthesizing Ag nanoparticles decorated carboxymethyl lignin (CML) functionalized Fe3O4 nanocomposite (Fe3O4@CML/Ag) in situ, and demonstrates its chemical and biological applications. The structural and physicochemical characteristics of this novel material were evaluated using various analytical techniques. The nanomaterial showed excellent catalytic activity for the N-acylation of different amines under solvent-free conditions, and it could be reused multiple times. Additionally, the material exhibited promising antioxidant and anticancer properties, as confirmed by DPPH and MTT assays, respectively. The results suggest that this nanocomposite has potential applications in biomedicine.