4.7 Article

Core-binding factor fusion downregulation of ADAR2 RNA editing contributes to AML leukemogenesis

Journal

BLOOD
Volume 141, Issue 25, Pages 3078-3090

Publisher

AMER SOC HEMATOLOGY
DOI: 10.1182/blood.2022015830

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Adenosine deaminases acting on RNA (ADAR) family of enzymes, especially ADAR2, play a role in multiple cancers. This study shows that ADAR2 is downregulated in certain types of acute myeloid leukemia (AML) and suppresses leukemogenesis in AML cells with t(8;21) or inv(16) translocations, through its RNA editing function.
Adenosine-to-inosine RNA editing, which is catalyzed by adenosine deaminases acting on RNA (ADAR) family of enzymes, ADAR1 and ADAR2, has been shown to contribute to multiple cancers. However, other than the chronic myeloid leukemia blast crisis, relatively little is known about its role in other types of hematological malignancies. Here, we found that ADAR2, but not ADAR1 and ADAR3, was specifically downregulated in the core -binding factor (CBF) acute myeloid leukemia (AML) with t(8;21) or inv(16) translocations. In t(8;21) AML, RUNX1-driven transcription of ADAR2 was repressed by the RUNX1-ETO additional exon 9a fusion protein in a dominant-negative manner. Further functional studies confirmed that ADAR2 could suppress leukemogenesis specifically in t(8;21) and inv16 AML cells dependent on its RNA editing capability. Expression of 2 exemplary ADAR2-regulated RNA editing targets coatomer subunit & alpha; and component of oligomeric Golgi complex 3 inhibits the clonogenic growth of human t(8;21) AML cells. Our findings support a hitherto, unappreciated mechanism leading to ADAR2 dysregulation in CBF AML and highlight the functional relevance of loss of ADAR2-mediated RNA editing to CBF AML.

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