4.8 Article

Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples

Journal

BIOSENSORS & BIOELECTRONICS
Volume 228, Issue -, Pages -

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115202

Keywords

SARS-CoV-2 spike binding aptamer; Particle display; Silver nanoforest; SERS-based aptasensor; Label -free SERS biosensor

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COVID-19, caused by SARS-CoV-2, has led to a global pandemic and significant economic and social disruptions. To control the virus spread, a rapid, accurate, and easy-to-use diagnostic platform for detecting SARS-CoV-2 variants of concern is necessary. Researchers developed an ultra-sensitive label-free aptasensor based on surface-enhanced Raman scattering, which successfully detected SARS-CoV-2, including the wild-type, Delta, and Omicron variants.
COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused an ongoing global pandemic with economic and social disruption. Moreover, the virus has persistently and rapidly evolved into novel lineages with mutations. The most effective strategy to control the pandemic is suppressing virus spread through early detection of infections. Therefore, developing a rapid, accurate, easy-to-use diagnostic platform against SARS-CoV-2 variants of concern remains necessary. Here, we developed an ultra-sensitive label-free surface-enhanced Raman scattering-based aptasensor as a countermeasure for the universal detection of SARSCoV-2 variants of concern. In this aptasensor platform, we discovered two DNA aptamers that enable binding to SARS-CoV-2 spike protein via the Particle Display, a high-throughput screening approach. These showed high affinity that exhibited dissociation constants of 1.47 & PLUSMN; 0.30 nM and 1.81 & PLUSMN; 0.39 nM. We designed a combination with the aptamers and silver nanoforest for developing an ultra-sensitive SERS platform and achieved an attomolar (10-18 M) level detection limit with a recombinant trimeric spike protein. Furthermore, using the intrinsic properties of the aptamer signal, we demonstrated a label-free aptasensor approach, enabling use without the Raman tag. Finally, our label-free SERS-combined aptasensor succeeded in detecting SARS-CoV-2 with excellent accuracy, even in clinical samples with variants of concern, including the wild-type, delta, and omicron variants.

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