Journal
BIOSENSORS & BIOELECTRONICS
Volume 227, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2023.115155
Keywords
Cell immunocapture; Microsystems; Rare cell population; Circulating endothelial cells; Circulating tumor cells
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Cell immunocapture microsystems are an emerging field with potential medical diagnostic applications for isolating and quantifying circulating rare cells (CRCs). A simple and robust stop-flow microsystem based on planar antibody-coated surfaces was designed and achieved up to 90% immunocapture efficiency of MCF-7 cells in whole blood. The results can contribute to the design of microsystems for the isolation and identification of rare cells from blood.
Cell immunocapture microsystems are a fast-emerging field with several potential medical diagnostic applica-tions. Isolation and quantification of circulating rare cells (CRCs) show great importance in the early stages of disease diagnostics and prognostics. Here, we present a simple and robust stop-flow microsystem (fabricated by a combination of glass microblasting and 3D printing) based on a planar antibody-coated surface that is effective in the immunocapture of the model as well as naturally occurring rare cells. A chip with a planar immunocapture channel working in the so-called stop-flow dynamic regime was designed to enable monitoring the efficiency of the cell capture by fluorescence microscopy. Up to 90% immunocapture efficiency of MCF-7 cells spiked into whole blood on CD326 antibody-coated planar surfaces was achieved. We discuss the role of the planar surface modifications, the influence of the set stop-flow dynamic conditions, and medium complexity on the efficiency of cell immunocapture. The presented results could be further employed in the design of microsystems for cell-size -independent isolation and identification of rare cells from blood.
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